Characterisation of the Prevailing Multidrug Strains from Surgical Wound Using 16S rRNA Sequencing Technique.

BACKGROUND

() is prevalent in hospital-acquired surgical wound infections. It exhibits both innate and acquired resistance to a broad range of antimicrobials and remains a principal problem in clinical practice.

METHODS

In total, 284 sterile surgical wound swabs (142 each) were collected from two government hospitals: Central Hospital Benin (CHB) and University of Benin Teaching Hospital (UBTH) in Benin City, Nigeria. spp. isolated from both hospitals were screened with eight different antibiotics by way of disk diffusion method. Polymerase chain reaction (PCR) amplification of 34 multiple drug-resistant isolates was carried out using genus-specific primer set on extracted genomic DNA for the identification of spp. and substituent 16S rRNA sequencing to determine the prevailing strains in the two locations.

RESULTS

Sixty-two spp. were isolated from the two locations (27 isolates from CHB and 35 isolates from the UBTH). Surgical wound infections screened with regularly used antibiotics revealed that 17 (62.9%) isolates from CHB and 20 (57.1%) isolates from UBTH were multiple drug resistant spp. PCR identification using spp. specific primer showed that 16 (94.1%) isolates from CHB and 18 (90%) isolates from UBTH were confirmed. The 16S DNA sequencing revealed that strain H25883 was dominant in both locations.

CONCLUSION

High antibiotic resistance among isolates was established in our study. PCR technique revealed a more reliable method of bacterial identification. H25883 strain of is the prevalent strain in both locations and it should be given attention in nosocomial surgical wound infections.