Am J Bot. 1998 May;85(5):681.
Nuclear DNA contents (4C) were estimated by Feulgen microdensitometry in 27 species of slipper orchids. These data and recent information concerning the molecular systematics of Cypripedioideae allow an interesting re-evaluation of karyotype and genome size variation among slipper orchids in a phylogenetic context. DNA amounts differed 5.7-fold, from 24.4 pg in Phragmipedium longifolium to 138.1 pg in Paphiopedilum wardii. The most derived clades of the conduplicate-leaved slipper orchids have undergone a radical process of genome fragmentation that is most parsimoniously explained by Robertsonian changes involving centric fission. This process seems to have occurred independently of genome size variation. However, it may reflect environmental or selective pressures favoring higher numbers of linkage groups in the karyotype.
通过 Feulgen 显微光度术，我们对 27 种拖鞋兰属植物的核 DNA 含量（4C）进行了估计。这些数据以及 Cypripedioideae 分子系统学的最新信息，使得我们能够在系统发育背景下，对拖鞋兰属植物的核型和基因组大小变异进行有趣的重新评估。DNA 含量差异达到了 5.7 倍，范围从 Phragmipedium longifolium 的 24.4pg 到 Paphiopedilum wardii 的 138.1pg。内卷叶拖鞋兰属植物中最衍生的分支经历了基因组碎片化的剧烈过程，这最合理的解释是涉及着丝粒分裂的罗伯逊易位。这个过程似乎与基因组大小的变化无关。然而，它可能反映了有利于染色体组中更多连锁群数量的环境或选择压力。
