Qin Shiyu, Wang Hanchen, Wang Miaomiao, Shao Bingyi, Ma Chongbo, Yang Boyun, Jin Xiaohua
State Key Laboratory of Plant Diversity and Specialty Crops, Institute of Botany, Chinese Academy of Sciences, Beijing, China.
School of Life Sciences, Nanchang University, Nanchang, 330031, China.
Funct Integr Genomics. 2025 Apr 30;25(1):96. doi: 10.1007/s10142-025-01596-z.
In this study, the mitogenomes of nine species in the subfamily Cypripedioideae were newly sequenced and assembled using both short and long reads for evolutionary analyses. Complete multi-chromosomal mitogenomes were obtained for Cypripedium subtropicum, C. henryi, Phragmipedium humboldtii, Phr. kovachii, and Paphiopedilum micranthum, and draft assemblies were obtained for four additional Paphiopedilum species. Thirty-nine protein-coding genes were annotated and shared in nine sampled species. sdh4 was discovered in all species of Cypripedioideae, and rpl10 was detected in four species of Paphiopedilum. These two genes might have been horizontally transferred from non-orchid plants at different times. Approximately 101 to 998 repeat sequences were identified with total lengths of 417,136 to 785,960 bp in the mitogenomes of Cypripedioideae. There were 634 and 662 RNA editing sites in C. subtropicum and Pa. gratrixianum, respectively, and C-to-U editing was dominant. The nad and ccm genes exhibited high frequencies of RNA editing. Our study revealed the complexity of orchid mitogenomes, including evidence for the horizontal transfer of rpl10 and sdh4.
在本研究中，对杓兰亚科9个物种的线粒体基因组进行了新的测序，并使用短读长和长读长进行组装以进行进化分析。获得了暖地杓兰、亨利杓兰、洪堡兜兰、科瓦奇兜兰和小花兜兰完整的多染色体线粒体基因组，并获得了另外4种兜兰属物种的草图组装。在9个采样物种中注释并共享了39个蛋白质编码基因。在所有杓兰亚科物种中都发现了sdh4，在4种兜兰属物种中检测到了rpl10。这两个基因可能在不同时间从非兰花植物水平转移而来。在杓兰亚科的线粒体基因组中鉴定出约101至998个重复序列，总长度为417,136至785,960 bp。暖地杓兰和格拉茨兜兰分别有634个和662个RNA编辑位点，且C到U的编辑占主导。nad和ccm基因表现出较高的RNA编辑频率。我们的研究揭示了兰花线粒体基因组的复杂性，包括rpl10和sdh4水平转移的证据。
