Department of Surgery, National Defense Medical College, 3-2, Namiki, Tokorozawa, Saitama 359-8513, Japan.
Am J Clin Pathol. 2011 Jul;136(1):138-44. doi: 10.1309/AJCP63NRBNGCTXVF.
To obtain the correlation between morphologic features in the invasive fronts of colorectal cancer (CRC) and L1 cell adhesion molecule (L1CAM) expression, 275 CRCs were assessed with L1CAM immunostaining and 29 CRCs were examined for L1CAM messenger RNA (mRNA) expression. Based on immunostaining, the positive rate of L1CAM expression increased according to the grade of tumor budding (P = .0002) and solid cancer nests (SCNs; P = .0046). L1CAM mRNA levels at the invasive front of the tumor were higher than those at the center of the tumor (median, 3.7-fold). The gap of L1CAM mRNA level between the invasive front and the central area was 7.3-fold in tumors having SCN lesions, whereas it was 1.9-fold in tumors having non-SCN lesions (P = .0004). L1CAM expression was correlated with nodal involvement in protein and mRNA levels (P = .0007 and P = .036, respectively). Tumor regulation of L1CAM expression is associated with morphologic features at the invasive front in CRC.
为了获得结直肠癌（CRC）浸润前沿的形态学特征与 L1 细胞黏附分子（L1CAM）表达之间的相关性，对 275 例 CRC 进行了 L1CAM 免疫染色，对 29 例 CRC 进行了 L1CAM 信使 RNA（mRNA）表达检测。基于免疫染色，L1CAM 表达的阳性率随着肿瘤芽生（P=.0002）和实体癌巢（SCN；P=.0046）程度的增加而增加。肿瘤浸润前沿的 L1CAM mRNA 水平高于肿瘤中心（中位数，3.7 倍）。在有 SCN 病变的肿瘤中，L1CAM mRNA 水平在侵袭前沿和中心区域之间的差距为 7.3 倍，而在无 SCN 病变的肿瘤中为 1.9 倍（P=.0004）。L1CAM 表达与蛋白和 mRNA 水平的淋巴结受累相关（P=.0007 和 P=.036）。肿瘤对 L1CAM 表达的调节与 CRC 浸润前沿的形态学特征有关。
