[奥布齐丹、菲诺普汀和Ca2+对用异丙肾上腺素激活的豚鼠离体心脏收缩-舒张过程中环磷酸腺苷（cAMP）水平的影响]

[The effect of obzidan, finoptin and Ca2+ on the cAMP level during contraction-relaxation of isolated heart of guinea pigs activated with isadrin].

作者信息

Laptev B I, Bardamova I B, Prokop'eva V D, Afanas'ev S A, Kenikh N I, Travkov Iu A

出版信息

Ukr Biokhim Zh (1978). 1990 May-Jun;62(3):111-5.

Abstract

The beta-adrenoceptor blockade, slow inward calcium current blockade and calcium concentration decrease in perfusion solution were studied for their effect on cAMP oscillations during cardiac cycle in the isadrin-activated isolated heart of guinea pig. Isadrin activation (3 X 10(-8) M) of beta-adrenoceptors caused an increase of the cAMP concentration by 50% during the contraction. All the actions decreased an average level of cyclonucleotide in the myocardium as compared with isadrin and what is more, obsidan (10(-6) M) removed the cAMP oscillations almost completely, the hypocalcium solution (0.15 mM Ca2+) decreased their amplitude and duration, finoptin (3 X 10(-8) M) qualitatively changed the oscillation profile. The results obtained make it possible to suggest, that a change in the concentration of ionized calcium in myoplasma is one of the reasons inducing changes in the cAMP during the cardiac cycle.

摘要

研究了β-肾上腺素能受体阻断、慢内向钙电流阻断以及灌注液中钙浓度降低对豚鼠异肾上腺素激活的离体心脏心动周期中cAMP振荡的影响。β-肾上腺素能受体的异肾上腺素激活（3×10⁻⁸ M）导致收缩期cAMP浓度增加50%。与异肾上腺素相比，所有这些作用均降低了心肌中环核苷酸的平均水平，此外，奥昔丹（10⁻⁶ M）几乎完全消除了cAMP振荡，低钙溶液（0.15 mM Ca²⁺）降低了其振幅和持续时间，菲诺普汀（3×10⁻⁸ M）使振荡曲线发生了质的变化。所得结果表明，肌浆中离子钙浓度的变化是心动周期中诱导cAMP变化的原因之一。