The Institute of Optics, University of Rochester, 275 Hutchinson Road, Rochester, New York 14627, USA.
Opt Lett. 2011 Jun 15;36(12):2221-3. doi: 10.1364/OL.36.002221.
In this Letter, we report for the first time (to our knowledge) in-vivo volumetric optical coherence microscopy images of skin epidermal cells. We achieved micrometer-class resolution, 2 μm laterally and axially, with an acquisition speed of 23 K A-scans/s and over 90 dB sensitivity to a depth of 1 mm by employing a custom, liquid-lens-based, dynamic-focusing objective, a broadband light source, and a custom, astigmatism-corrected Czerny-Turner spectrometer with a high-speed complementary metal-oxide-semiconductor camera.
在这封信件中，我们首次（据我们所知）报告了活体皮肤表皮细胞的体积光学相干显微镜图像。我们通过采用定制的、基于液体透镜的动态聚焦物镜、宽带光源和具有高速互补金属氧化物半导体相机的定制消像散 Czerny-Turner 光谱仪，实现了横向和轴向 2μm 的亚微米级分辨率、23 K 次/秒的采集速度和超过 90 dB 的灵敏度，可探测深度达 1mm。
