Department of Otorhinolaryngology, University of Schleswig-Holstein Campus Lübeck, 23538 Lübeck, Germany.
Oncol Rep. 2011 Sep;26(3):615-20. doi: 10.3892/or.2011.1350. Epub 2011 Jun 17.
Human plasmacytoid dendritic cells (PDCs) are present in solid tumor tissue and metastatic cervical lymph nodes (CLN) in head and neck squamous cell carcinoma (HNSCC). We recently showed that classical PDC functions are heavily disturbed in the tumor microenvironment. In this study we present a new approach to the subject by introducing 3 PDC subsets in HNSCC, characterized by the surface markers CD25, CD56 and CD203c. The first subset, positive for CD25, is significantly induced by HNSCC in vitro and present in metastatic lymph nodes in vivo. This subset can be phenotypically subdivided into matured cells and into a group expressing early T cell markers. Functionally this subgroup is associated with the secretion of IL-8. The second subset, positive for CD56, constitutes 4-5% of all PDCs and is significantly down-regulated by HNSCC. Furthermore, this population sporadically expresses perforin/granzyme B and is absent in metastatic lymph nodes. The third subset, positive for the basophile marker CD203c, is inducible by crosslinking BDCA-2 in the presence of HNSCC and IL-4. Future studies will have to clarify the in vivo relevance of the different PDC subsets in HNSCC.
人类浆细胞样树突状细胞（PDC）存在于头颈部鳞状细胞癌（HNSCC）的实体瘤组织和转移性颈淋巴结（CLN）中。我们最近表明，PDC 的经典功能在肿瘤微环境中受到严重干扰。在这项研究中，我们通过引入 HNSCC 中的 3 种 PDC 亚群，即 CD25、CD56 和 CD203c 表面标志物，为该主题提供了一种新方法。第一个亚群 CD25 阳性，在体外由 HNSCC 显著诱导，并存在于体内转移性淋巴结中。该亚群可以表型细分为成熟细胞和表达早期 T 细胞标志物的细胞群。功能上，该亚群与 IL-8 的分泌有关。第二个亚群 CD56 阳性，占所有 PDC 的 4-5%，被 HNSCC 显著下调。此外，该群体偶尔表达穿孔素/颗粒酶 B 并且不存在于转移性淋巴结中。第三个亚群 CD203c 阳性，是在存在 HNSCC 和 IL-4 的情况下通过交联 BDCA-2 诱导的。未来的研究将不得不阐明不同的 PDC 亚群在 HNSCC 中的体内相关性。
