College of Animal Science and Technology, Northwest A&F University, Shaanxi Key Laboratory of Molecular Biology for Agriculture, Yangling, 712100 Shaanxi, China.
Mol Biol Rep. 2012 Mar;39(3):2305-11. doi: 10.1007/s11033-011-0980-2. Epub 2011 Jun 18.
Retinoic acid receptor responder 2 gene (RARRES2) encodes a novel adipokine protein that plays a crucial role in regulating several biological processes, including immune responses, adipocyte differentiation, type 2 diabetes and metabolic syndrome. In this paper, polymorphisms of the bovine RARRES2 gene were detected in 1300 individuals from six breeds by DNA pooling, CRS-PCR-RFLP and DNA sequencing methods. The results showed that NC_007302:g.117035859A>G, 117035706G>A and 117034290A>G were in the coding region, which resulted in three synonymous mutations and only 117033779C>G was in the 3' UTR. Additionally, associations of the four novel SNPs with growth traits were analyzed in Nanyang cattle up to 2 years of age. In P1-PvuII locus, individuals with genotype BC had greater body height and hucklebone width than those with genotype AA, AC and AB at the age of 24 months. In P3-BamHI locus, individuals with genotype AG had higher hucklebone width than those with genotype GG at the age of 24 months. However, no statistically significant differences were observed in P5-SmaI locus. These results indicated that RARRES2 gene might be a potential candidate gene for marker-assisted selection (MAS).
维甲酸受体应答基因 2（RARRES2）编码一种新型脂肪因子蛋白，在调节包括免疫反应、脂肪细胞分化、2 型糖尿病和代谢综合征在内的多种生物学过程中发挥着关键作用。在本研究中，采用 DNA 池、CRS-PCR-RFLP 和 DNA 测序方法对来自六个品种的 1300 个个体的牛 RARRES2 基因多态性进行了检测。结果表明，NC_007302：g.117035859A>G、117035706G>A 和 117034290A>G 位于编码区，导致三个同义突变，只有 117033779C>G 位于 3'UTR。此外，还分析了这四个新的 SNP 与南阳牛 2 岁以下生长性状的相关性。在 P1-PvuII 位点，24 月龄时，基因型 BC 的个体比基因型 AA、AC 和 AB 的个体具有更高的体高和髋骨宽度。在 P3-BamHI 位点，24 月龄时，基因型 AG 的个体比基因型 GG 的个体具有更高的髋骨宽度。然而，在 P5-SmaI 位点未观察到统计学上的显著差异。这些结果表明，RARRES2 基因可能是一个用于标记辅助选择（MAS）的潜在候选基因。
