Division of Pharmacology and ESR Laboratories, Department of Clinical Care Medicine, Kanagawa Dental College, 82 Inaoka-cho, Yokosuka, Kanagawa, 238-8580, Japan.
J Anesth. 2011 Oct;25(5):765-9. doi: 10.1007/s00540-011-1184-6. Epub 2011 Jun 18.
Some antioxidant anesthetics directly inhibit lipid peroxidation mediated via the generation of reactive oxygen species (ROS). To date, the scavenging effects of midazolam on ROS have not been directly assessed. We investigated the inhibitory effect of midazolam on ROS [hydroxyl radical (HO(·)) and superoxide (O (2) (·-) )] by in vitro X-band electron spin resonance with the spin-trapping agent 5,5-dimethyl-1-pyrroline-N-oxide. Our results indicated that HO(·) and O (2) (·-) were not affected by midazolam at clinically relevant concentrations, but were directly scavenged by midazolam at high concentrations (i.e., >4.6 and >1.5 mM, respectively).
一些抗氧化麻醉剂可直接抑制通过活性氧（ROS）产生介导的脂质过氧化。迄今为止，咪达唑仑对 ROS 的清除作用尚未直接评估。我们通过体外 X 波段电子自旋共振和自旋捕获剂 5,5-二甲基-1-吡咯啉-N-氧化物研究了咪达唑仑对 ROS [羟基自由基（HO(·)）和超氧阴离子（O (2) (·-) ）]的抑制作用。我们的结果表明，HO(·)和 O (2) (·-)在临床相关浓度下不受咪达唑仑影响，但在高浓度下（即分别>4.6 和>1.5 mM）被咪达唑仑直接清除。
