Komatsu Tomoko, Lee Masaichi-Chang-il, Miyagi Atsushi, Shoji Hirofumi, Yoshino Fumihiko, Maehata Yojiro, Maetani Takashi, Kawamura Yosuke, Ikeda Masakazu, Kubota Eiro
Department of Clinical Care Medicine, Division of Dentistry for Special Patients, Kanagawa Dental College, Kanagawa, Japan.
Redox Rep. 2006;11(2):71-7. doi: 10.1179/135100006X101039.
Oral manifestations of Down syndrome include high susceptibility to gingival inflammation with early onset and rapidly progressive periodontitis. The influence of reactive oxygen species (ROS) on periodontitis of Down syndrome is unclear. The aim of this study was to characterize ROS formation in Down syndrome-gingival fibroblasts (DS-GF) using electron spin resonance (ESR) spin trapping with 5,5-dimetyl-1-pyrolline-N-oxide (DMPO), and to determine whether ROS generation plays a role in the pathogenesis of periodontitis in Down syndrome patients. We observed formation of the DMPO-OH spin adduct, indicating HO* generation from cultured DS-GF and non-DS-GF. The increased HO* generation in cultured DS-GF was strongly decreased in the presence of the H2O2 scavenger, catalase, or the iron chelator, desferal. This may due to the enzymatic ability of over-expressed CuZn-superoxide dismutase in Down syndrome to catalyze the formation of H2O2 from O2*-, thereby increasing the availability of substrate H2O2 for the iron-dependent generation of HO* via the Fenton reaction, suggesting that HO* generated from DS-GF may be involved in progressive periodontitis of Down syndrome.
唐氏综合征的口腔表现包括对牙龈炎症高度易感，发病早且牙周炎进展迅速。活性氧（ROS）对唐氏综合征牙周炎的影响尚不清楚。本研究的目的是使用5,5 - 二甲基 - 1 - 吡咯啉 - N - 氧化物（DMPO）通过电子自旋共振（ESR）自旋捕获技术来表征唐氏综合征牙龈成纤维细胞（DS - GF）中ROS的形成，并确定ROS生成是否在唐氏综合征患者牙周炎的发病机制中起作用。我们观察到了DMPO - OH自旋加合物的形成，表明培养的DS - GF和非DS - GF产生了羟基自由基（HO*）。在过氧化氢清除剂过氧化氢酶或铁螯合剂去铁胺存在的情况下，培养的DS - GF中增加的HO生成显著减少。这可能是由于唐氏综合征中过度表达的铜锌超氧化物歧化酶的酶促能力，可催化由超氧阴离子（O2 - ）形成过氧化氢（H2O2），从而增加了通过芬顿反应铁依赖性生成HO的底物H2O2的可用性，这表明DS - GF产生的HO可能参与了唐氏综合征的进行性牙周炎。
