Kirino H, Kuwahara R, Hamasaki N, Oshima T
Department of Life Science, Tokyo Institute of Technology, Kanagawa.
J Biochem. 1990 May;107(5):661-5. doi: 10.1093/oxfordjournals.jbchem.a123104.
The effect of unusual polyamines, such as thermine, caldopentamine, caldohexamine, tris(3-aminopropyl)amine, or tetrakis(3-aminopropyl)ammonium, on the activities of various restriction endonucleases was investigated by using an Escherichia coli plasmid as a substrate, which contains a high GC content fragment from an extreme thermophile. Restriction enzymes used were SmaI, BanII, NaeI, RsaI, and TaqI. Most of the polyamines tested were inhibitory to the enzyme activities. The larger and more branched a polyamine was, the more the activities of nucleases were inhibited. The inhibition was positively correlated with the polyamine concentration. The sites protected by a polyamine were identical to those protected by other polyamines, and also identical to those which were less sensitive to the restriction enzyme in the absence of polyamines. No sequence specificity was seen among these sites.
以含有来自极端嗜热菌的高GC含量片段的大肠杆菌质粒为底物，研究了诸如热胺、卡尔多戊胺、卡尔多己胺、三（3-氨丙基）胺或四（3-氨丙基）铵等异常多胺对各种限制性内切酶活性的影响。所使用的限制性酶为SmaI、BanII、NaeI、RsaI和TaqI。测试的大多数多胺对酶活性具有抑制作用。多胺越大且分支越多，核酸酶的活性受到的抑制就越大。抑制作用与多胺浓度呈正相关。一种多胺保护的位点与其他多胺保护的位点相同，也与在不存在多胺时对限制性酶不太敏感的位点相同。在这些位点之间未观察到序列特异性。
