Uzawa T, Yamagishi A, Nishikawa K, Oshima T
Department of Life Science, Tokyo Institute of Technology, Yokohama.
J Biochem. 1994 May;115(5):830-2. doi: 10.1093/oxfordjournals.jbchem.a124424.
The effects of novel polyamines on aminoacyl-tRNA formation catalyzed by Escherichia coli., Sulfolobus acidocaldarius, and Thermus thermophilus HB8 S-100 extracts were investigated. These effects were diverse and differed depending on the amino acid and the enzyme used. A quaternary polyamine, tetrakis(3-aminopropyl)ammonium, inhibited phenylalanyl-tRNA synthesis catalyzed by the T. thermophilus extract, but not inhibit the other aminoacyl-tRNA formations tested. The inhibition was observed in hybrid reactions where the thermophile tRNA or extract was replaced by its E. coli counterpart, although the quaternary amine did not inhibit Phe-tRNA formation by the E. coli homologous system. Spermine relieved the inhibition of the reaction of thermophile enzyme and tRNA, but not the inhibition of the hybrid reactions. These results suggest that the branched polyamine interacts with both the thermophile enzyme and tRNA(Phe).
研究了新型多胺对大肠杆菌、嗜酸热硫化叶菌和嗜热栖热菌HB8 S-100提取物催化的氨酰-tRNA形成的影响。这些影响是多样的，并且因所使用的氨基酸和酶而异。一种季铵多胺，四（3-氨丙基）铵，抑制嗜热栖热菌提取物催化的苯丙氨酰-tRNA合成，但不抑制所测试的其他氨酰-tRNA的形成。在杂交反应中观察到了抑制作用，其中嗜热菌的tRNA或提取物被其大肠杆菌对应物取代，尽管季铵胺不抑制大肠杆菌同源系统的苯丙氨酰-tRNA形成。精胺缓解了嗜热菌酶和tRNA反应的抑制，但没有缓解杂交反应的抑制。这些结果表明，支链多胺与嗜热菌酶和tRNA（苯丙氨酸）都相互作用。
