Department of Biomedical Engineering, Faculty of Engineering, Tel Aviv University, Tel Aviv, Israel.
Biophys J. 2011 Jun 22;100(12):2855-64. doi: 10.1016/j.bpj.2011.04.040.
Nasal epithelial cells secret mucins and are exposed in vivo to airflow-induced mechanophysical stresses, including wall shear stress (WSS), temperature, and humidity. In this work, human nasal epithelial cells cultured under air-liquid interface conditions were subjected to fields of airflow-induced oscillatory WSS at different temperature and humidity conditions. Changes in mucin secretion due to WSS were measured and the role of the cytoskeleton in mucin secretion was explored. Mucin secretion significantly increased in response to WSS in a magnitude-dependent manner with respect to static cultures and independently of the airflow temperature and humidity. In static cultures, mucin secretion decreased at high humidity with or without elevation of the temperature with respect to cultures at a comfortable climate. In cultures exposed to WSS, mucin secretion increased at high temperature with respect to cultures at comfortable climate conditions. The polymerization of actin microfilaments was shown to increase mucin secretion under WSS, whereas the dynamics of microtubule polymerization did not affect secretion. In conclusion, the data in this study show that mucin secretion is sensitive to oscillatory WSS as well as high temperature and humidity conditions.
鼻腔上皮细胞分泌粘蛋白，并在体内暴露于气流引起的机械物理应激下，包括壁面切应力（WSS）、温度和湿度。在这项工作中，在气液界面条件下培养的人鼻腔上皮细胞在不同的温度和湿度条件下受到气流诱导的振荡 WSS 场的作用。由于 WSS 导致的粘蛋白分泌的变化被测量，并且细胞骨架在粘蛋白分泌中的作用被探索。粘蛋白分泌在很大程度上依赖于静态培养物的静态培养物，并且独立于气流的温度和湿度，与 WSS 呈依赖性增加。在静态培养物中，与在舒适气候条件下的培养物相比，高湿度下粘蛋白分泌减少，而温度升高或降低则没有影响。在暴露于 WSS 的培养物中，与在舒适气候条件下的培养物相比，高温下粘蛋白分泌增加。微丝聚合被证明在 WSS 下增加粘蛋白分泌，而微管聚合动力学不影响分泌。总之，本研究的数据表明，粘蛋白分泌对振荡 WSS 以及高温和高湿度条件敏感。
