生物聚集体抗真菌活性的体外评估。

An in vitro evaluation of antifungal activity of bioaggregate.

作者信息

Dohaithem Abdullah, Al-Nasser Abdulrahman, Al-Badah Abdulhakim, Al-Nazhan Saad, Al-Maflehi Nassr

机构信息

King Fahd Armed Forces Hospital, Jeddah, Saudi Arabia.

出版信息

Oral Surg Oral Med Oral Pathol Oral Radiol Endod. 2011 Oct;112(4):e27-30. doi: 10.1016/j.tripleo.2011.03.037.

DOI:10.1016/j.tripleo.2011.03.037

PMID:21689954

Abstract

OBJECTIVE

The aim of this study was to evaluate, in vitro, the antifungal effect of bioaggregate (BA) against Candida albicans by using the direct contact method.

STUDY DESIGN

BA was tested freshly mixed and after 24-hour set on C. albicans. The tested BA was incubated with C. albicans in plastic tissue clusters for 1 hour, 24 hours, and 3 and 5 days. Aliquots of 0.1 mL were taken from each well at the end of the incubation periods and transfered to tubes containing 5 mL fresh Sabouraud broth. All tubes were vortexed and then incubated at 37°C and observed for the subsequent 5 days. Growth of the fungi was observed daily by the presence of turbidity in the tubes. The results were statistically analyzed by using Kaplan-Meier test.

RESULTS

The freshly mixed and set BA had no antifungal effect at 1 and 24 hours of contact. Both mixes demonstrated complete fungicidal activity after 24 hours' contact. Statistical analysis showed a highly significant difference between the negative and positive control groups (P < .001) and a significant difference between the freshly mixed and 24-hour set BA groups (P < .001) at 24 hours.

CONCLUSIONS

BA (freshly mixed and 24-hour set) was effective against C. albicans after 24 hours.

摘要

目的

本研究旨在通过直接接触法在体外评估生物聚集体（BA）对白色念珠菌的抗真菌作用。

研究设计

对新鲜混合的BA以及放置24小时后的BA进行针对白色念珠菌的测试。将测试的BA与白色念珠菌在塑料组织培养簇中孵育1小时、24小时、3天和5天。在孵育期结束时，从每个孔中取出0.1 mL的等分试样，转移至含有5 mL新鲜沙氏肉汤的试管中。将所有试管涡旋振荡，然后在37°C下孵育，并在随后的5天内进行观察。通过观察试管中是否出现浑浊来每日监测真菌的生长情况。使用Kaplan-Meier检验对结果进行统计学分析。