Mohammadi Zahed, Modaresi Jalil, Yazdizadeh Mohammad
Department of Endodontics, Shahid Sadoughi University of Medical Sciences, Yazd, Iran.
Aust Endod J. 2006 Dec;32(3):120-2. doi: 10.1111/j.1747-4477.2006.00032.x.
The aim of this study was to evaluate and compare the antifungal effect of white-coloured mineral trioxide aggregate (WMTA) and grey mineral trioxide aggregate (GMTA) using a tube-dilution test. MTA preparations were freshly mixed and tested after 24 h on Candida albicans. The experiment was carried out in 24-well culture plates. Fifty wells were used and divided into four experimental groups (freshly mixed WMTA, freshly mixed GMTA, 24 h-set WMTA and 24 h-set GMTA) of 10 wells each and control groups of five wells each. Plates of Sabouraud dextrose agar mixed with C. albicans served as positive control, and Sabouraud dextrose agar without C. albicans served as negative control. Fresh inoculate of C. albicans was prepared by growing an overnight culture from a stock culture. Aliquots of C. albicans were then taken from the stock culture and plated on the agar compound of the experimental and control groups. All plates were incubated at 37 degrees C for 1, 24 and 72 h. Growth of fungi was monitored daily by the presence of turbidity. Results showed that in the freshly mixed as well as 24 h-set MTA cements fungal growth was observed during 1-h incubation, whereas by increasing the incubation time, no fungal growth was observed in 24 and 72 h. It was concluded that MTA cements (freshly mixed and 24-h set) were effective against C. albicans.
本研究旨在通过试管稀释试验评估并比较白色矿物三氧化物凝聚体（WMTA）和灰色矿物三氧化物凝聚体（GMTA）的抗真菌效果。MTA制剂新鲜混合后，在对白色念珠菌作用24小时后进行测试。实验在24孔培养板中进行。使用50个孔，分为四个实验组（新鲜混合的WMTA、新鲜混合的GMTA、凝固24小时的WMTA和凝固24小时的GMTA），每组10个孔，以及每组5个孔的对照组。与白色念珠菌混合的沙氏葡萄糖琼脂平板用作阳性对照，不含白色念珠菌的沙氏葡萄糖琼脂平板用作阴性对照。通过从储备培养物中培养过夜培养物来制备白色念珠菌的新鲜接种物。然后从储备培养物中取出白色念珠菌的等分试样，接种到实验组和对照组的琼脂混合物上。所有平板在37℃下孵育1、24和72小时。通过观察浑浊情况每天监测真菌生长。结果表明，在新鲜混合以及凝固24小时的MTA水门汀中，孵育1小时期间观察到真菌生长，而随着孵育时间延长，在24小时和72小时未观察到真菌生长。得出的结论是，MTA水门汀（新鲜混合和凝固24小时）对白色念珠菌有效。
