Julius Wolff Institute, Charité-Universitätsmedizin Berlin, Berlin, Germany.
Ann Biomed Eng. 2011 Oct;39(10):2531-41. doi: 10.1007/s10439-011-0338-x. Epub 2011 Jun 21.
Mechanobiological theories have been introduced to illustrate the interaction between biology and the local mechanical environment during bone healing. Although several theories have been proposed, a quantitative validation using histomorphometric data is still missing. In this study, in vivo histological data based on an ovine animal experiment was quantified and used to validate bone healing simulations focussing on the endochondral ossification process. The bone formation at different callus regions (periosteal and endosteal bone at the medial and lateral side) was analyzed from in vivo data and quantitatively compared with in silico results. A histomorphometric difference was found in medial and lateral hard callus formation 3 weeks after osteotomy. However, the same amount of new bone was formed on both sides between week 3 and 6. Using a parametric approach, distinct ranges for mechanical strain levels regulating tissue formation were found, for which the in silico prediction agrees with the in vivo endochondral ossification both in pattern and quantity. According to this finding, a strain range of 1 to 8% seems to be conducive for cartilage formation while bone formation may be facilitated by strains up to 4%. This study demonstrates the potential of a thorough validation of in silico results for gaining a better understanding of mechanobiological mechanisms during bone healing.
力学生物学理论被引入来解释骨骼愈合过程中生物学与局部力学环境之间的相互作用。尽管已经提出了几种理论，但仍然缺乏使用组织形态计量学数据进行定量验证。在这项研究中，使用基于绵羊动物实验的体内组织学数据进行了量化，并用于验证专注于软骨内成骨过程的骨骼愈合模拟。从体内数据中分析了不同骨痂区域（内侧和外侧的骨膜和骨内膜）的骨形成，并与体内结果进行了定量比较。在截骨后 3 周时，发现内侧和外侧硬骨痂形成存在组织学差异。然而，在第 3 周到第 6 周之间，两侧形成了相同数量的新骨。使用参数方法，发现了调节组织形成的机械应变水平的明显范围，体内软骨内成骨的体内预测在模式和数量上与体内预测一致。根据这一发现，1%至 8%的应变范围似乎有利于软骨形成，而高达 4%的应变可能有利于骨形成。这项研究表明，对体内结果进行彻底验证具有潜力，可以更好地理解骨骼愈合过程中的力学生物学机制。
