Department of Biomedical Engineering, Vanderbilt University, 2201 West End Ave, Nashville, TN 37235.
Department of Chemical and Biomolecular Engineering, Vanderbilt University, 2201 West End Ave, Nashville, TN 37235.
ACS Biomater Sci Eng. 2020 Jan 13;6(1):564-574. doi: 10.1021/acsbiomaterials.9b01378. Epub 2019 Dec 10.
Resorbable bone cements are replaced by bone osteoclastic resorption and osteoblastic new bone formation near the periphery. However, the ideal bone cement would be replaced by new bone through processes similar to fracture repair, which occurs through a variable combination of endochondral and intramembranous ossification. In this study, nanocrystalline hydroxyapatite (nHA)-poly(thioketal urethane) (PTKUR) cements were implanted in femoral defects in New Zealand White rabbits to evaluate ossification at 4, 12, and 18 months. Four formulations were tested: an injectable, flowable cement and three moldable putties with varying ratios of calcium phosphate to sucrose granules. New bone formation and resorption of the cement by osteoclasts occurred near the periphery. Stevenel's Blue and Safranin O staining revealed infiltration of chondrocytes into the cements and ossification of the cartilaginous intermediate. These findings suggest that nHA-PTKUR cements support combined intramembranous and endochondral ossification, resulting in enhanced osseointegration of the cement that could potentially improve patient outcomes.
可吸收骨水泥在其周围被破骨细胞吸收和成骨细胞新骨形成所替代。然而，理想的骨水泥将通过类似于骨折修复的过程被新骨替代，后者通过膜内成骨和软骨内成骨的不同组合发生。在这项研究中，纳米晶羟基磷灰石(nHA)-聚(硫代缩酮氨酯)(PTKUR)骨水泥被植入新西兰白兔的股骨缺损中，以评估在 4、12 和 18 个月时的成骨情况。测试了四种配方：一种可注射的、可流动的水泥和三种具有不同钙磷比和蔗糖颗粒比的可模塑油灰。在其周围，新骨形成和破骨细胞对水泥的吸收。Stevenel 蓝和番红 O 染色显示软骨细胞渗透到水泥中，并发生软骨内骨化。这些发现表明 nHA-PTKUR 骨水泥支持膜内和软骨内成骨的联合，从而增强了水泥的骨整合，有可能改善患者的预后。
