National Laboratory for Molecular Sciences, Center for Molecular Sciences, State Key Laboratory for Structural Chemistry of Unstable and Stable Species, Institute of Chemistry, Chinese Academy of Sciences, Beijing, PR China.
Biochimie. 2011 Aug;93(8):1351-6. doi: 10.1016/j.biochi.2011.06.007. Epub 2011 Jun 17.
Both G-quadruplexes and natural polyamines are intimately associated with tumor growth and proliferation. The effect of the natural polyamines on telomeric and some oncogenic G-quadruplexes including bcl-2, c-kit, and c-myc G-quadruplexes has been studied by using absorption, fluorescence, CD, and NMR methods. The results exhibited an interesting dual-effect depending on polyamineś concentration. Polyamines promote and stabilize G-quadruplexes under a lower concentration (less than 1mM) but denature G-quadruplexes under a higher concentration (more than 1mM). Probably the electrostatic and hydrophobic effect of polyamines and the hydrogen-bonding interaction between guanines and polyamines were respectively responsible for the stabilizing and denaturing effect.
G-四链体和天然多胺都与肿瘤的生长和增殖密切相关。通过吸收、荧光、CD 和 NMR 方法研究了天然多胺对端粒和一些致癌 G-四链体（包括 bcl-2、c-kit 和 c-myc G-四链体）的影响。结果显示，多胺的浓度依赖性表现出一种有趣的双重效应。在较低浓度（小于 1mM）下，多胺促进和稳定 G-四链体，但在较高浓度（大于 1mM）下，多胺使 G-四链体变性。可能是多胺的静电和疏水效应以及鸟嘌呤和多胺之间的氢键相互作用分别负责稳定和变性效应。
