Wu L, Franke J
Department of Anatomy and Cell Biology, College of Physicians and Surgeons of Columbia University, New York, NY 10032.
Gene. 1990 Jul 2;91(1):51-6. doi: 10.1016/0378-1119(90)90161-j.
A 1.6-kb genomic fragment containing the coding region for the inhibitor (PDI) of cyclic nucleotide phosphodiesterase (PD) was isolated and sequenced. The genomic sequence includes 510 nucleotides (nt) of 5'-noncoding sequence and the full coding sequence, which contains two small introns. From the deduced amino acid (aa) sequence we predict a 26-kDa protein that, in agreement with previous data, contains approximately 15% Cys residues. The PDI possesses a hydrophobic leader sequence, five potential glycosylation sites, and three internal repeats. Northern-blot analysis showed a single transcript of 0.95 kb. The gene encoding PDI (pdi) was expressed early in development with little transcript remaining following aggregation. The appearance of pdi transcript was inhibited by cAMP, but when cAMP was removed the transcript appeared within 30 min. When cAMP was applied to cells containing pdi mRNA, the transcript disappeared with a half-life of less than 30 min.
分离并测序了一个1.6kb的基因组片段，该片段包含环核苷酸磷酸二酯酶（PD）抑制剂（PDI）的编码区。基因组序列包括510个核苷酸（nt）的5'非编码序列和完整的编码序列，其中包含两个小内含子。从推导的氨基酸（aa）序列中，我们预测出一个26kDa的蛋白质，与先前的数据一致，该蛋白质含有约15%的半胱氨酸残基。PDI具有一个疏水前导序列、五个潜在的糖基化位点和三个内部重复序列。Northern印迹分析显示有一个0.95kb的单一转录本。编码PDI（pdi）的基因在发育早期表达，聚集后几乎没有转录本残留。pdi转录本的出现受到cAMP的抑制，但当去除cAMP时，转录本在30分钟内出现。当将cAMP应用于含有pdi mRNA的细胞时，转录本以小于30分钟的半衰期消失。
