Barklis E, Pontius B, Lodish H F
Mol Cell Biol. 1985 Jun;5(6):1473-9. doi: 10.1128/mcb.5.6.1473-1479.1985.
The gene encoding the prestalk D11 mRNA of Dictyostelium discoideum has been isolated and characterized. Transcriptional mapping and sequence data indicated that the D11 message is unspliced and contains an 846-base open reading frame. The 273 base pairs upstream from the translation initiator codon are 88% A + T, typical of Dictyostelium upstream sequences, and contain no recognizable upstream activator sequence. The deduced D11 protein is exceptionally rich in cysteine residues and consists of a 25-amino acid hydrophobic leader sequence (L) followed by a series of repeats with the structure LA1B1A2B2C1B3C2B4C3B5C4B6, where A, B, and C, are, respectively, amino acid sequences of 39, 18, and 15 residues. The deduced D11 protein shares certain similarities with the Dictyostelium cyclic AMP phosphodiesterase inhibitor protein.
编码盘基网柄菌前柄D11 mRNA的基因已被分离和鉴定。转录图谱和序列数据表明，D11信使RNA未被剪接，包含一个846个碱基的开放阅读框。翻译起始密码子上游的273个碱基对中A+T含量为88%，这是盘基网柄菌上游序列的典型特征，且不包含可识别的上游激活序列。推导的D11蛋白富含半胱氨酸残基，由一个25个氨基酸的疏水前导序列（L）组成，后面跟着一系列具有LA1B1A2B2C1B3C2B4C3B5C4B6结构的重复序列，其中A、B和C分别是39、18和15个残基的氨基酸序列。推导的D11蛋白与盘基网柄菌环磷酸腺苷磷酸二酯酶抑制蛋白有一定的相似性。
