Wyatt L S, Balachandran N, Frenkel N
Laboratory of Viral Diseases, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Rockville, Maryland 20852.
J Infect Dis. 1990 Oct;162(4):852-7. doi: 10.1093/infdis/162.4.852.
The Z29 and U1102 strains of human herpesvirus 6 (HHV-6) were compared for their ability to replicate in fresh peripheral blood lymphocytes (PBL) and in continuous T cell lines. The replication of both strains in PBL was enhanced by mitogenic activation of cell growth. U1102 replicated in the continuous T cell lines, J JHAN and HSB-2, whereas no Z29 replication was observed in these cell lines as judged by infectious virus yields, the presence of viral antigens, and viral DNA replication. The two strains were compared with respect to their ability to react in immunofluorescence assays with monoclonal antibodies (MAbs) prepared against the GS strain of HHV-6. These MAbs are directed against six different polypeptides including three glycoproteins. All MAbs reacted with cells infected with the U1102 strain. The Z29-infected cells reacted with four MAbs but failed to react with MAbs specific for an 82- to 105-kDa major surface glycoprotein and with one MAb reactive with a nonglycosylated 180-kDa protein. Taken together, the two strains of HHV-6 exhibit variations with regard to their growth and antigenic properties.
对人疱疹病毒6型（HHV - 6）的Z29和U1102毒株在新鲜外周血淋巴细胞（PBL）和连续T细胞系中的复制能力进行了比较。两种毒株在PBL中的复制通过细胞生长的促有丝分裂激活得以增强。U1102在连续T细胞系JJHAN和HSB - 2中复制，而根据感染性病毒产量、病毒抗原的存在以及病毒DNA复制判断，在这些细胞系中未观察到Z29的复制。就它们与针对HHV - 6 GS毒株制备的单克隆抗体（MAb）在免疫荧光试验中的反应能力对这两种毒株进行了比较。这些MAb针对六种不同的多肽，包括三种糖蛋白。所有MAb都与感染U1102毒株的细胞发生反应。感染Z29的细胞与四种MAb发生反应，但不与针对82至105 kDa主要表面糖蛋白的MAb以及与一种与非糖基化180 kDa蛋白反应的MAb发生反应。综上所述，HHV - 6的这两种毒株在生长和抗原特性方面表现出差异。
