Ablashi D V, Balachandran N, Josephs S F, Hung C L, Krueger G R, Kramarsky B, Salahuddin S Z, Gallo R C
Laboratory of Cellular and Molecular Biology, National Cancer Institute, Bethesda, Maryland 20892.
Virology. 1991 Oct;184(2):545-52. doi: 10.1016/0042-6822(91)90424-a.
Fifteen human herpesvirus-6 (HHV-6) isolates from normal donors and patients with AIDS, systemic lupus erythematosis, chronic fatigue syndrome, collagen-vascular disease, leukopenia, bone marrow transplants, Exanthem subitum (roseola), and atypical polyclonal lymphoproliferation were studied for their tropism to fresh human cord blood mononuclear cells, growth in continuous T cell lines, reactivity to monoclonal antibodies, and by restriction enzyme banding patterns. All isolates replicated efficiently in human cord blood mononuclear cells, but mitogen stimulation of the cells prior to infection was required. The ability to infect continuous T-cell lines varied with the isolates. Isolates similar to GS prototype infected HSB2 and Sup T1 cells and did not infect Molt-3 cells, whereas isolates similar to Z-29 infected Molt-3 cells but not HSB2 and Sup T1 cells. Some of the monoclonal antibodies directed against the HHV-6 (GS) isolate showed reactivity with all isolates tested, but others only reacted with HHV-6 isolates similar to the GS isolate and not with those similar to Z-29 isolate. Restriction enzyme analysis using EcoRI, BamHI, and HindIII revealed that HHV-6 isolates from roseola, bone marrow transplant, leukopenia, and an HIV-1-positive AIDS patient from Zaire (Z-29) were closely related but distinct from GS type HHV-6 isolates. Based on the above findings, we propose that, like herpes simplex virus types 1 and 2, the 15 HHV-6 isolates analyzed can be divided into group A (GS type) and group B (Z-29 type).
对来自正常供体以及患有艾滋病、系统性红斑狼疮、慢性疲劳综合征、胶原血管疾病、白细胞减少症、骨髓移植、幼儿急疹（玫瑰疹）和非典型多克隆淋巴细胞增殖症患者的15株人类疱疹病毒6型（HHV-6）分离株，研究了它们对新鲜人脐血单个核细胞的嗜性、在连续T细胞系中的生长情况、对单克隆抗体的反应性以及限制性酶切图谱。所有分离株都能在人脐血单个核细胞中高效复制，但感染前需要对细胞进行丝裂原刺激。感染连续T细胞系的能力因分离株而异。与GS原型相似的分离株能感染HSB2和Sup T1细胞，但不能感染Molt-3细胞，而与Z-29相似的分离株能感染Molt-3细胞，但不能感染HSB2和Sup T1细胞。一些针对HHV-6（GS）分离株的单克隆抗体与所有测试的分离株都有反应，但其他一些仅与类似于GS分离株的HHV-6分离株反应，而不与类似于Z-29分离株的反应。使用EcoRI、BamHI和HindIII进行的限制性酶切分析显示，来自玫瑰疹、骨髓移植、白细胞减少症患者以及来自扎伊尔的一名HIV-1阳性艾滋病患者（Z-29）的HHV-6分离株密切相关，但与GS型HHV-6分离株不同。基于上述发现，我们提出，与1型和2型单纯疱疹病毒一样，所分析的15株HHV-6分离株可分为A组（GS型）和B组（Z-29型）。
