Campadelli-Fiume G, Guerrini S, Liu X, Foà-Tomasi L
Department of Experimental Pathology, University of Bologna, Italy.
J Gen Virol. 1993 Oct;74 ( Pt 10):2257-62. doi: 10.1099/0022-1317-74-10-2257.
The distribution of glycoprotein B (gB) among different human herpesvirus 6 (HHV-6) strains was analysed with a panel of three monoclonal antibodies (MAbs) derived from mice immunized with U1102-infected lymphocytes. MAb 2D9 reacted specifically by immunofluorescence and immunoprecipitation with the U1102 and GS isolates, and failed to react with Z29 and the variant B strains Hashimoto and SF. In addition, Z29, Hashimoto and SF gB had a lower M(r) than U1102 and GS gB. MAb 2D9 also failed to react with the exanthem subitum isolate CV, included in this study as an as yet poorly characterized isolate. Consistent with this result, CV failed to react with the variant A-specific MAb to gp82-105 and behaved as a variant B virus even with respect to the diagnostic HindIII endonuclease restriction cleavage site located in a fragment hybridizing to the pZVH14 probe. By contrast with MAb 2D9, MAbs 2B9 and 2D10 reacted with all of the isolates tested, strengthening the argument tha they have common epitopes. Based on the antigenic and M(r) specificities of gB, the HHV-6 isolates tested were arranged into two non-overlapping clusters, which closely parallel the variant A and B strain groups, defined previously by several criteria, including restriction endonuclease polymorphism, antigenic variations, growth in in vitro cultures and sequence analyses.
用一组由感染U1102的淋巴细胞免疫的小鼠产生的三种单克隆抗体（MAb），分析了糖蛋白B（gB）在不同人类疱疹病毒6型（HHV-6）毒株中的分布情况。单克隆抗体2D9通过免疫荧光和免疫沉淀反应，与U1102和GS分离株发生特异性反应，但不与Z29以及变异B毒株桥本和SF发生反应。此外，Z29、桥本和SF的gB分子量低于U1102和GS的gB。单克隆抗体2D9也不与本研究中作为特征尚不明确的分离株纳入的幼儿急疹分离株CV发生反应。与这一结果一致，CV不与针对gp82 - 105的变异A特异性单克隆抗体发生反应，并且即使对于位于与pZVH14探针杂交的片段中的诊断性HindIII内切酶限制性切割位点而言，其表现也与变异B病毒相同。与单克隆抗体2D9形成对比的是，单克隆抗体2B9和2D10与所有测试的分离株都发生反应，这进一步证明它们具有共同的表位。基于gB的抗原性和分子量特异性，所测试的HHV-6分离株被分为两个不重叠的簇，这与先前通过包括限制性内切酶多态性、抗原变异、体外培养生长和序列分析等多种标准定义的变异A和变异B毒株组密切平行。
