Department of Gastroenterology and Hepatology, Zhongshan Hospital, Fudan University, Shanghai 200032, People's Republic of China.
Mol Cell Biochem. 2011 Dec;358(1-2):53-60. doi: 10.1007/s11010-011-0920-4. Epub 2011 Jun 22.
The effect of curcumin on liver injury caused by Concanavalin A (Con A) has not been carefully examined. This study was designed to evaluate the protective effect of curcumin on Con A-induced hepatitis in mice. Liver injured mice received curcumin by gavage at a dose of 200 mg/kg body weight before Con A intravenous administration. Curcumin was effective in reducing the elevated plasma levels of aminotransferases and the incidence of liver necrosis compared with Con A-injected control group. Enzyme-linked immunosorbent assay (ELISA) showed that curcumin suppressed proinflammatory cytokines such as tumor necrosis factor (TNF)-α, interferon (IFN)-γ, and interleukin (IL)-4 production in Con A-injected mice. The reduced severity of hepatitis in curcumin pretreated mice correlated with decrease in numbers of liver CD4(+) T cells but not CD8(+) T cells by immunohistochemical analysis. Furthermore, the expression levels of intercellular adhesion molecule-1 (ICAM-1) and the interferon-inducible chemokine CXCL10 in hepatic tissue were significantly decreased by curcumin pretreatment. In conclusion, curcumin pretreatment protects against T cell-mediated hepatitis in mice.
姜黄素对刀豆蛋白 A (Con A) 诱导的肝损伤的作用尚未被仔细研究。本研究旨在评估姜黄素对 Con A 诱导的小鼠肝炎的保护作用。肝损伤小鼠在 Con A 静脉注射前通过灌胃给予 200mg/kg 体重的姜黄素。与 Con A 注射对照组相比，姜黄素有效降低了升高的血浆转氨酶水平和肝坏死的发生率。酶联免疫吸附试验 (ELISA) 显示，姜黄素抑制了促炎细胞因子如肿瘤坏死因子 (TNF)-α、干扰素 (IFN)-γ 和白细胞介素 (IL)-4 在 Con A 注射小鼠中的产生。姜黄素预处理小鼠肝炎严重程度降低与肝 CD4(+) T 细胞数量减少有关，但与 CD8(+) T 细胞无关，通过免疫组织化学分析。此外，细胞间黏附分子-1 (ICAM-1) 和干扰素诱导的趋化因子 CXCL10 的表达水平在肝组织中也明显降低。综上所述，姜黄素预处理可预防小鼠 T 细胞介导的肝炎。
