Zhu Lianhua, Cho Eunae, Zhao Guohua, Roh Mi Ryung, Zheng Zhenlong
Department of Dermatology, Yanbian University Hospital, Yanji, P.R. China.
Department of Oral Pathology, Oral Cancer Research Institute, Yonsei University College of Dentistry, Seoul, Republic of Korea.
In Vivo. 2019 Mar-Apr;33(2):393-400. doi: 10.21873/invivo.11486.
BACKGROUND/AIM: Cortactin (CTTN) has been considered a promising molecular prognostic factor in various types of cancers. In this study, we aimed to investigate the role of CTTN in the pathogenesis of cutaneous squamous cell carcinoma (CSCC).
CTTN and phospho-CTTN (p-CTTN) expression was determined in 10 healthy controls and 38 CSCC tissue samples by immunohistochemistry. The influence of CTTN on the biological behavior of CSCC cells was also investigated.
p-CTTN expression was significantly increased in CSCC than control samples. In contrast, no significant difference in CTTN expression was found between control and CSCC tissues. Moreover, a significant association was found between recurrence-free survival with p-CTTN expression, but not with CTTN expression. Furthermore, the proliferative, migratory, and invasive abilities of CSCC cells were significantly decreased by CTTN-siRNA transfection.
CTTN phosphorylation is strongly associated with CSCC pathogenesis and may serve as a molecular biomarker of CSCC.
背景/目的：在各类癌症中，皮层肌动蛋白（CTTN）被视为一种颇具前景的分子预后因素。在本研究中，我们旨在探究CTTN在皮肤鳞状细胞癌（CSCC）发病机制中的作用。
采用免疫组织化学法，在10例健康对照者及38例CSCC组织样本中检测CTTN和磷酸化CTTN（p-CTTN）的表达。同时也研究了CTTN对CSCC细胞生物学行为的影响。
与对照样本相比，CSCC中p-CTTN表达显著增加。相比之下，对照组织与CSCC组织之间CTTN表达无显著差异。此外，无复发生存率与p-CTTN表达之间存在显著关联，但与CTTN表达无关。此外，CTTN-siRNA转染显著降低了CSCC细胞的增殖、迁移和侵袭能力。
CTTN磷酸化与CSCC发病机制密切相关，可能作为CSCC的分子生物标志物。
