Department of Radiology, Yonsei University College of Medicine, Seoul 120-752, Republic of Korea.
Biomaterials. 2011 Sep;32(26):6174-82. doi: 10.1016/j.biomaterials.2011.04.007. Epub 2011 Jun 21.
The synchronization of gene expression and cell trafficking in transfected stem cells is crucial for augmentation of stem cell functions (differentiation and neurotropic factor secretion) and real time in vivo monitoring. We report a magnetic nanoparticle-based gene delivery system that can ensure simultaneous gene delivery and in vivo cell trafficking by high resolution MR imaging. The polar aprotic solvent soluble MnFe₂O₄ nanoparticles were enveloped using cationic polymers (branched polyethyleneimine, PEI) by the solvent shifting method for a gene loading. Using our magnetic nanovector system (PEI-coated MnFe₂O₄ nanoparticles), thus, we synchronized stem cell migration and its gene expression in a rat stroke model.
转染干细胞中基因表达和细胞迁移的同步对于增强干细胞功能（分化和神经营养因子分泌）和实时体内监测至关重要。我们报告了一种基于磁性纳米粒子的基因传递系统，该系统可以通过高分辨率磁共振成像确保基因传递和体内细胞迁移的同时进行。通过溶剂转移法，用阳离子聚合物（支化聚乙烯亚胺，PEI）包裹非质子极性溶剂可溶性 MnFe₂O₄ 纳米粒子，以进行基因负载。因此，使用我们的磁性纳米载体系统（PEI 包覆的 MnFe₂O₄ 纳米粒子），我们在大鼠中风模型中同步了干细胞的迁移及其基因表达。
