Department of Chemical and Biomolecular Engineering, College of Engineering, Yonsei University, Seoul 120-749, Republic of Korea.
Nanoscale Res Lett. 2013 Sep 26;8(1):399. doi: 10.1186/1556-276X-8-399.
Nucleic acid-based aptamers have been developed for the specific delivery of diagnostic nanoprobes. Here, we introduce a new class of smart imaging nanoprobe, which is based on hybridization of a magnetic nanocrystal with a specific aptamer for specific detection of the angiogenic vasculature of glioblastoma via magnetic resonance (MR) imaging. The magnetic nanocrystal imaging core was synthesized using the thermal decomposition method and enveloped by carboxyl polysorbate 80 for water solubilization and conjugation of the targeting moiety. Subsequently, the surface of the carboxylated magnetic nanocrystal was modified with amine-functionalized aptamers that specifically bind to the vascular growth factor receptor 2 (VEGFR2) that is overexpressed on angiogenic vessels. To assess the targeted imaging potential of the aptamer-conjugated magnetic nanocrystal for VEGFR2 markers, the magnetic properties and MR imaging sensitivity were investigated using the orthotopic glioblastoma mouse model. In in vivo tests, the aptamer-conjugated magnetic nanocrystal effectively targeted VEGFR2 and demonstrated excellent MR imaging sensitivity with no cytotoxicity.
核酸适配体已被开发用于诊断纳米探针的特异性递送。在这里，我们介绍了一类新的智能成像纳米探针，它基于磁性纳米晶体与特定适配体的杂交，通过磁共振 (MR) 成像特异性检测神经胶质瘤的血管生成血管。磁性纳米晶体成像核心使用热分解法合成，并通过羧基聚山梨酯 80 包被以实现水溶解和靶向部分的缀合。随后，羧基化磁性纳米晶体的表面用胺功能化的适配体修饰，该适配体特异性结合在血管生成血管上过度表达的血管内皮生长因子受体 2 (VEGFR2)。为了评估适配体偶联的磁性纳米晶体对 VEGFR2 标志物的靶向成像潜力，使用原位神经胶质瘤小鼠模型研究了磁性纳米晶体的磁性和磁共振成像灵敏度。在体内试验中，适配体偶联的磁性纳米晶体有效地靶向 VEGFR2 ，并表现出优异的磁共振成像灵敏度，没有细胞毒性。
