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通过 DART 质谱法在固体载体上检测共价结合底物的通用方法。

Versatile method for the detection of covalently bound substrates on solid supports by DART mass spectrometry.

机构信息

Department of Chemistry and Biochemistry, University of California Santa Cruz, Santa Cruz, California 95064, USA.

出版信息

Org Lett. 2011 Aug 5;13(15):3770-3. doi: 10.1021/ol201404v. Epub 2011 Jun 24.

DOI:10.1021/ol201404v
PMID:21699263
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC3146587/
Abstract

Analysis of substrates directly on solid phase resins without the need for separate cleavage conditions remains an outstanding challenge in the field of solid phase synthesis. We now present the first example of simultaneous cleavage and mass spectrometric analysis of peptides from solid supports using direct analysis in real time (DART) mass spectrometry. We have shown that this method is compatible with a diverse array of solid phase resins and is suitable for analysis of both peptides and organic substrates.

摘要

在固相合成领域，无需单独的裂解条件即可直接在固相树脂上分析底物仍然是一个突出的挑战。我们现在首次提出了使用实时直接分析(DART)质谱法直接从固相载体上同时裂解和分析肽的方法。我们已经证明，该方法与多种固相树脂兼容，适用于分析肽和有机底物。

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