1型单纯疱疹病毒株包膜多肽变体的凝胶过滤高效液相色谱分析
Gel filtration high performance liquid chromatography of envelope polypeptide variants of herpes simplex type 1 strains.
作者信息
al-Ahdal M N, Hussain Qadri S M, McGarry T J
机构信息
Department of Biological and Medical Research, King Faisal Specialist Hospital and Research Centre, Riyadh, Saudi Arabia.
出版信息
Biomed Chromatogr. 1990 Jul;4(4):148-51. doi: 10.1002/bmc.1130040406.
High performance liquid chromatography (HPLC) with a TSK-4000SW gel filtration column was used to compare envelope polypeptides from four strains of herpes simplex virus type 1 (HSV-1). The chromatographic profiles demonstrated polypeptide variability among three clinical strains and the wild-type F strain. Radioimmunoprecipitation of the HPLC fractions with polyclonal anti-HSV-1 followed by SDS-polyacrylamide gel electrophoresis (PAGE) of the immunoprecipitates revealed molecular weight differences of various polypeptides in fractions from the area containing major peaks. This HPLC method could prove useful for the analysis of polypeptide polymorphism in clinical isolates of HSV-1, as well as in other viruses.
采用配备TSK - 4000SW凝胶过滤柱的高效液相色谱法（HPLC）对四株1型单纯疱疹病毒（HSV - 1）的包膜多肽进行比较。色谱图谱显示了三株临床分离株与野生型F株之间多肽的变异性。用多克隆抗HSV - 1对HPLC级分进行放射免疫沉淀，然后对免疫沉淀物进行十二烷基硫酸钠 - 聚丙烯酰胺凝胶电泳（PAGE），结果显示来自主峰区域级分中各种多肽的分子量存在差异。这种HPLC方法可能对分析HSV - 1临床分离株以及其他病毒中的多肽多态性有用。
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