al-Ahdal M N
Department of Biological and Medical Research, King Faisal Specialist Hospital and Research Centre, Riyadh, Kingdom of Saudi Arabia.
Microbios. 1993;74(299):95-102.
Three clinical strains (isolates 1174, 1857, and 1937) of herpes simplex virus type 1 (HSV-1) were compared with the standard laboratory strain F of HSV-1 with respect to glycoprotein content in order to examine intratypic differences. Variation was demonstrated by radioimmunoprecipitation (RIP) with monoclonal antibodies to individual HSV-1 glycoproteins in purified virus particles and in infected cells. Compared with strain F, there was an increase in the concentrations of gC, gH, and gI in strain 1174, in gC and gH in strain 1857, and in gE and gG in strain 1937. The concentration of glycoproteins in purified virus particles from all strains was also compared to corresponding concentrations in infected cells by RIP. Levels lower in infected cells than in purified virus particles were observed for gG, gH and gI of the F strain, for gB, gC and gD of strain 1174, for gD, gG and gI of strain 1857, and for gC, gD, gE, gH and gI of strain 1937. The differences in viral protein processing and the heterogeneity observed in HSV-1 strains are discussed.
为了研究1型单纯疱疹病毒（HSV-1）的型内差异，将3株临床分离株（分离株1174、1857和1937）与HSV-1的标准实验室菌株F在糖蛋白含量方面进行了比较。通过用针对纯化病毒颗粒和感染细胞中单个HSV-1糖蛋白的单克隆抗体进行放射免疫沉淀（RIP）来证明差异。与菌株F相比，菌株1174中gC、gH和gI的浓度增加，菌株1857中gC和gH的浓度增加，菌株1937中gE和gG的浓度增加。还通过RIP将所有菌株纯化病毒颗粒中糖蛋白的浓度与感染细胞中的相应浓度进行了比较。F菌株的gG、gH和gI、菌株1174的gB、gC和gD、菌株1857的gD、gG和gI以及菌株1937的gC、gD、gE、gH和gI在感染细胞中的水平低于纯化病毒颗粒中的水平。讨论了HSV-1菌株中观察到的病毒蛋白加工差异和异质性。
