Research Laboratory of Comparative Biochemistry and Molecular Biology, Ternopil National Pedagogical University, M. Kryvonosa Street 2, Ternopil 46027, Ukraine.
Comp Biochem Physiol C Toxicol Pharmacol. 2011 Sep;154(3):242-53. doi: 10.1016/j.cbpc.2011.06.001. Epub 2011 Jun 15.
The aim of this study was to compare the effect of pro-oxidants copper (Cu(2+), 0.005 and 0.050mg L(-1)) or manganese (Mn(2+), 0.17 and 1.7mg L(-1)) on Carassius auratus gibelio from polluted (B) and unpolluted (Z) sites after exposure for fourteen days. Fish from site B showed high levels of lipid peroxidation (TBARS concentration), lower levels of metallothionein (MT)-related metal, total glutathione (GSH), its redox index, superoxide dismutase (Cu,Zn- and Mn-SOD), glutathione-S-transferase (GST) and cholinesterase (ChE) activities and also higher MT-related thiol concentration in the liver and gills. A common effect of exposure was related to genotoxicity, a decrease in GSH and an increase in microsomal ethoxyresorufin O-deethylase activity in the liver. However, the systems of oxidative stress and biotransformation were more efficient in fish from the polluted site, while the responsivity of MTs in this fish was impaired. Principle Component Analysis separated the subgroups from the unpolluted site and fish loaded by lesser concentrations of metals on the one side, and fish from the polluted site jointly with fish exposed to higher concentrations of metals on the other side. The main distinguishing indices of sites and exposures selected by classification and regression tree (CART) analysis were MT characteristics and genotoxicity.
本研究旨在比较氧化剂铜（Cu(2+)，0.005 和 0.050mg/L）或锰（Mn(2+)，0.17 和 1.7mg/L）对来自污染（B）和未污染（Z）地点的鲫鱼（Carassius auratus gibelio）的影响，暴露时间为 14 天。B 点的鱼类表现出高水平的脂质过氧化（TBARS 浓度）、低水平的金属硫蛋白（MT）相关金属、总谷胱甘肽（GSH）、其氧化还原指数、超氧化物歧化酶（Cu、Zn 和 Mn-SOD）、谷胱甘肽-S-转移酶（GST）和胆碱酯酶（ChE）活性，以及肝脏和鳃中 MT 相关巯基浓度较高。暴露的一个共同影响与遗传毒性有关，即 GSH 减少和肝脏微粒体乙氧基色原 O-脱乙基酶活性增加。然而，在污染地点的鱼类中，氧化应激和生物转化系统更为有效，而这种鱼类的 MT 反应能力受损。主成分分析将未污染地点和鱼类的亚组与受较少浓度金属加载的鱼类以及受较高浓度金属暴露的鱼类分开。分类和回归树（CART）分析选择的地点和暴露的主要鉴别指标是 MT 特征和遗传毒性。
