Research Laboratory of Comparative Biochemistry and Molecular Biology, Ternopil National Pedagogical University, 2, M. Kryvonosa Str. Ternopil, 46027, Ukraine.
Comp Biochem Physiol C Toxicol Pharmacol. 2012 Mar;155(2):396-406. doi: 10.1016/j.cbpc.2011.11.001. Epub 2011 Nov 18.
The aim of this study was to evaluate population-related peculiarities of the adaptive responses of Carassius auratus gibelio. In order to do this, male specimens from polluted (B) and clean (Z) sites were exposed to commercial pesticides thiocarbamate Tatoo (9.1 μg·L(-1)and 91 μg·L(-1)) or tetrazine Apollo (2 μg·L(-1) and 10 μg·L(-1)) during fourteen days. The control fish from site B was distinguished by weakness of antioxidant defence (measured from superoxide dismutase and catalase activities, redox index of glutathione (GSH), superoxide anion (O(2)) and lipid peroxidation levels), imbalance of the concentrations of protein metallothionein (MT-SH) and MT-related metals (MT-Me) and neurotoxicity. Differences in glutathione-S-transferase activity in the liver and vitellogenin-like proteins in the serum were also showed between B and Z control groups. Common effects of pesticides were related to a decrease in GSH, an increase in O(2) production, ethoxyresorufin O-deethylase activity and hepatosomatic index. Apollo provoked particular elevation of MT-SH/MT-Me ratio. Population-related difference in the response was the activation of antioxidant defence in fish from site B and its inhibition in fish from site Z. The genotoxic effect of exposures was more expressed in fish from site B. Principal component analysis combine all exposed groups from site Z and control group from site B in one set, and separated each exposed group from site B. The main distinguishing index of each population selected by classification and regression tree analysis was MT-SH.
本研究旨在评估鲫（Carassius auratus gibelio）适应反应的种群特有特性。为此，在 14 天内，将来自污染（B）和清洁（Z）地点的雄性标本暴露于商业杀虫剂硫代氨基甲酸酯 Tatoo（9.1μg·L（-1）和 91μg·L（-1））或四嗪 Apollo（2μg·L（-1）和 10μg·L（-1））中。来自 B 点的对照鱼表现出抗氧化防御能力减弱（通过超氧化物歧化酶和过氧化氢酶活性、谷胱甘肽（GSH）的氧化还原指数、超氧阴离子（O（2））和脂质过氧化水平来衡量）、蛋白金属硫蛋白（MT-SH）和 MT 相关金属（MT-Me）浓度失衡以及神经毒性。B 和 Z 对照组之间还显示了肝脏谷胱甘肽-S-转移酶活性和血清卵黄蛋白原样蛋白的差异。杀虫剂的共同作用与 GSH 减少、O（2）产生增加、乙氧基resorufin O-脱乙基酶活性和肝体比增加有关。Apollo 引起 MT-SH/MT-Me 比值特别升高。种群相关的反应差异是 B 点鱼的抗氧化防御激活和 Z 点鱼的抗氧化防御抑制。暴露的遗传毒性作用在 B 点鱼中更为明显。主成分分析将 Z 点的所有暴露组和 B 点的对照组结合在一起，将 B 点的每个暴露组分开。分类和回归树分析选择的每个种群的主要鉴别指数是 MT-SH。
