Liu Hui, Wang Weimu, Zhang JingFei, Wang XiaoRong
Department of Agricultural Engineering, Hohai University, Nanjing 210098, People's Republic of China.
Ecotoxicol Environ Saf. 2006 Nov;65(3):350-4. doi: 10.1016/j.ecoenv.2005.09.002. Epub 2005 Oct 24.
Copper bioavailability in the tissues of goldfish and antioxidant defenses in the liver of fish were investigated in vivo following 40 days of exposure to different species of copper solutions at different concentrations. Copper seemed to be more concentrated in the gill than in the liver after Cu(2+) and copper-ethylenediaminetetraacetate (Cu-EDTA) exposure. The addition of EDTA markedly affected copper adsorption in the gill but had no significant effects in the liver. We also found that in the fish exposed to the concentration of Cu(2+) below the Chinese national fishery water quality standard of 0.01 mg/L, superoxide dismutase (SOD) and glutathione S-transferase (GST) activities in liver were stimulated and catalase (CAT) and Se-dependent glutathione peroxidase (Se-GPx) activities were inhibited after 40 days of exposure. Compared to copper exposure, the addition of EDTA did not affect SOD and GST significantly. However, CAT and GPx activity with Cu(2+) exposure were significantly different from those with Cu-EDTA exposure.
在金鱼体内，研究了在40天内暴露于不同浓度的不同种类铜溶液后，金鱼组织中的铜生物有效性以及鱼肝脏中的抗氧化防御。在暴露于Cu(2+)和乙二胺四乙酸铜（Cu-EDTA）后，鳃中的铜似乎比肝脏中更富集。添加乙二胺四乙酸（EDTA）显著影响鳃中铜的吸附，但对肝脏没有显著影响。我们还发现，在暴露于低于中国国家渔业水质标准0.01 mg/L的Cu(2+)浓度的鱼中，暴露40天后，肝脏中的超氧化物歧化酶（SOD）和谷胱甘肽S-转移酶（GST）活性受到刺激，而过氧化氢酶（CAT）和硒依赖性谷胱甘肽过氧化物酶（Se-GPx）活性受到抑制。与铜暴露相比，添加EDTA对SOD和GST没有显著影响。然而，Cu(2+)暴露组的CAT和GPx活性与Cu-EDTA暴露组的显著不同。
