非洲爪蟾卵母细胞提取物介导的DNA超螺旋化：对一种核因子的需求
DNA supercoiling by Xenopus laevis oocyte extracts: requirement for a nuclear factor.
作者信息
Baldi M I, Mattoccia E, Tocchini-Valentini G P
出版信息
Proc Natl Acad Sci U S A. 1978 Oct;75(10):4873-6. doi: 10.1073/pnas.75.10.4873.
A purified system is described for the introduction of negative supercoils into simian virus 40 DNA. The system consists of histones H2A, H2B, H3, and H4, DNA-relaxing enzyme, and a purified factor from Xenopus laevis stage 6 oocyte nuclei. The nuclei are prepared en masse by the technique of F. Scalenghe, M. Buscaglia, C. Steinheil, and M. Crippa [(1978) Chromosoma 60, 299-308]. The supercoiled simian virus 40 DNA prepared by this method is indistinguishable from simian virus 40 supercoiled DNA prepared from infected monkey cells.
描述了一种用于将负超螺旋引入猿猴病毒40 DNA的纯化系统。该系统由组蛋白H2A、H2B、H3和H4、DNA松弛酶以及来自非洲爪蟾第6期卵母细胞核的一种纯化因子组成。细胞核通过F.斯卡伦盖、M.布斯卡利亚、C.施泰因海伊尔和M.克里帕的技术大规模制备[(1978)《染色体》60, 299 - 308]。用这种方法制备的超螺旋猿猴病毒40 DNA与从感染猴细胞中制备的猿猴病毒40超螺旋DNA无法区分。
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