Cooper L C, Dial E J, Lichtenberger L M
Department of Physiology and Cell Biology, University of Texas Medical School, Houston 77225.
Dig Dis Sci. 1990 Oct;35(10):1211-6. doi: 10.1007/BF01536409.
Ulcerogenesis of the duodenal mucosa frequently involves an inflammatory reaction with infiltration of leukocytes. Measurement of neutrophil myeloperoxidase activity might thus be a sensitive indicator of damage, before visible lesions occur. To test this possibility, a rat model for duodenal injury was used where fasted animals were treated with indomethacin and histamine-diHCl. Twenty-four hours after indomethacin treatment, duodenal tissues were collected for histochemical staining and biochemical assay for myeloperoxidase activity. Indomethacin- and histamine-challenged rats had significantly elevated myeloperoxidase activity compared to unchallenged controls (P less than 0.05) for both histochemistry and biochemistry. There was also a significant correlation between these two parameters (r = 0.68, P less than 0.001). The duodenal injury model then was used to test the effectiveness of known gastric protective agents. Results indicated that milk and buttermilk did not aggravate or protect against duodenal injury, while antacid and prostaglandin did significantly protect against inflammation (P less than 0.02). We concluded that measurement of myeloperoxidase activity is a sensitive and potentially useful estimate of duodenal injury that can be valuable in assessing ulcerogenesis and healing.
十二指肠黏膜溃疡形成常常涉及伴有白细胞浸润的炎症反应。因此，在可见病变出现之前，测量中性粒细胞髓过氧化物酶活性可能是损伤的一个敏感指标。为了验证这种可能性，使用了一种十二指肠损伤大鼠模型，对禁食动物用吲哚美辛和组胺二盐酸盐进行处理。吲哚美辛处理24小时后，收集十二指肠组织进行组织化学染色和髓过氧化物酶活性的生化测定。对于组织化学和生物化学检测，与未受刺激的对照组相比，经吲哚美辛和组胺刺激的大鼠髓过氧化物酶活性显著升高（P小于0.05）。这两个参数之间也存在显著相关性（r = 0.68，P小于0.001）。然后使用十二指肠损伤模型来测试已知胃保护剂的有效性。结果表明，牛奶和酪乳既不会加重也不会预防十二指肠损伤，而抗酸剂和前列腺素确实能显著预防炎症（P小于0.02）。我们得出结论，髓过氧化物酶活性的测量是十二指肠损伤的一种敏感且可能有用的评估方法，在评估溃疡形成和愈合方面可能具有重要价值。
