Department of Anesthesiology and Medical Crisis Management, Nagoya City University Graduate School of Medical Sciences, Nagoya, Japan.
JPEN J Parenter Enteral Nutr. 2011 Jul;35(4):516-22. doi: 10.1177/0148607110381599.
Inflammatory or oxidative stress is related to various diseases, including not only inflammatory diseases, but also diabetes, cancer, and atherosclerosis. The aim of this study was to evaluate the anti-inflammatory effects of a new enteral diet, MHN-02, which contains abundant antioxidants and whey peptide. The study also investigated the ability of MHN-02 to attenuate lethality, liver injury, the production of inflammatory cytokines, and the production of oxidized products using a carbon tetrachloride-induced rat model of severe fulminant hepatitis.
Male Sprague-Dawley rats were fed either a control diet or the MHN-02 diet for 14 days and injected with 2 mL/kg of carbon tetrachloride. Survival of rats was monitored from day 0 to day 3. To evaluate liver injury, inflammation, and oxidative stress, blood and liver samples were collected, and aspartate aminotransferase, alanine aminotransferase, lactate dehydrogenase, interleukin 6, tumor necrosis factor-α, and superoxide dismutase activity as a free radical scavenger were measured. A portion of the liver was evaluated histologically.
The survival rates of rats receiving the MHN-02 diet and the control diet were 90% and 55%, respectively. In the MHN-02 diet group, levels of serum liver enzymes and serum cytokines were significantly lower than in the control group. Superoxide dismutase activity in the MHN-02 diet was significantly higher in the MHN-02 group. Pathological lesions were significantly larger in the control group.
Supplementation of enteral diets containing whey peptide and antioxidants may protect against severe hepatitis.
炎症或氧化应激与各种疾病有关，不仅包括炎症性疾病，还包括糖尿病、癌症和动脉粥样硬化。本研究旨在评估一种新型肠内营养配方 MHN-02 的抗炎作用，该配方富含抗氧化剂和乳清肽。该研究还使用四氯化碳诱导的大鼠暴发性肝炎模型，研究了 MHN-02 减轻死亡率、肝损伤、炎症细胞因子产生和氧化产物产生的能力。
雄性 Sprague-Dawley 大鼠分别用对照饮食或 MHN-02 饮食喂养 14 天，并注射 2mL/kg 的四氯化碳。从第 0 天到第 3 天监测大鼠的存活情况。为了评估肝损伤、炎症和氧化应激，采集血液和肝脏样本，并测量天冬氨酸转氨酶、丙氨酸转氨酶、乳酸脱氢酶、白细胞介素 6、肿瘤坏死因子-α和超氧化物歧化酶活性作为自由基清除剂。部分肝脏进行组织学评估。
接受 MHN-02 饮食和对照饮食的大鼠的存活率分别为 90%和 55%。在 MHN-02 饮食组中，血清肝酶和血清细胞因子水平明显低于对照组。MHN-02 饮食组的超氧化物歧化酶活性明显更高。对照组的病理损伤明显更大。
补充含有乳清肽和抗氧化剂的肠内饮食可能对重症肝炎有保护作用。
