Inui Toshio, Kawamura Namiko, Yamamura Masahiro, Kubo Kentaro, Yamakage Hajime, Satoh-Asahara Noriko, Ogawa Yoshihiro, Katsuura Goro
Saisei Mirai Cell Processing Center, Moriguchi, Japan.
Cancer Immunotherapy Clinic, 6-14-17 Kinda-cho, Moriguchi-shi, Osaka, 570-0011, Japan.
Sci Rep. 2024 Dec 28;14(1):30859. doi: 10.1038/s41598-024-81597-3.
In order to elucidate novel actions of degalactosylated whey protein (D-WP) in comparison with intact whey protein (WP), the effects of oral intake of D-WP on peripheral blood telomere length and telomerase were examined in young and aged mice. In young mice, peripheral blood telomere length was significantly elongated following oral intake of D-WP for 4 weeks. mRNA expression of both telomerase reverse transcriptase (TERT) and telomerase RNA component (TERC) was significantly increased in the peripheral blood following oral intake of D-WP for 4 weeks. In aged mice, peripheral blood telomere length was significantly decreased as compared with that of young mice, and significantly restored to the level of young mice drinking water by the oral intake of D-WP for 4 weeks. The mRNA expression of peripheral blood TERT and TERC mRNA in aged mice significantly decreased as compared with the level in young mice drinking water, and was significantly restored to the level of expression of young mice drinking water by oral intake of D-WP for 4 weeks. These results suggest that D-WP, but not WP, potently increases peripheral blood telomere length accompanied by increased mRNA expression of TERT and TERC in both young and aged mice.
为了阐明去半乳糖基化乳清蛋白（D-WP）与完整乳清蛋白（WP）相比的新作用，研究了在年轻和老年小鼠中口服D-WP对外周血端粒长度和端粒酶的影响。在年轻小鼠中，口服D-WP 4周后外周血端粒长度显著延长。口服D-WP 4周后，外周血中端粒酶逆转录酶（TERT）和端粒酶RNA组分（TERC）的mRNA表达均显著增加。在老年小鼠中，外周血端粒长度与年轻小鼠相比显著缩短，口服D-WP 4周后显著恢复到饮用纯水的年轻小鼠的水平。老年小鼠外周血TERT和TERC mRNA的表达与饮用纯水的年轻小鼠相比显著降低，口服D-WP 4周后显著恢复到饮用纯水的年轻小鼠的表达水平。这些结果表明，在年轻和老年小鼠中，D-WP而非WP能有效增加外周血端粒长度，并伴随TERT和TERC的mRNA表达增加。
