Guo Ting-ting, Xu Hong-li, Zhang Lu-xi, Zhang Jian-peng, Guo Yi-feng, Gu Jia-wen, He Pei-min
Department of Aqua-Life Science and Technology, Shanghai Fisheries University, Shanghai 200090, China.
Regul Toxicol Pharmacol. 2007 Nov;49(2):101-6. doi: 10.1016/j.yrtph.2006.11.009. Epub 2007 Feb 14.
To study the protective effect and possible mechanism of Porphyra yezoensis polysaccharide (PYP) in hepatotoxicity mice, acute liver injury was successfully induced by injecting 0.2% carbon tetrachloride (CCl(4)) intraperitoneally. Levels of alanine aminotransferase (ALT) and aspartate aminotransferase (AST) in serum and liver homogenate, content of malondialdehyde (MDA), activities of total superoxide dismutase (T-SOD) in liver were measured by biochemical methods. Liver index was calculated and pathological changes of the liver tissue were observed microscopically. PYP was found to significantly decrease the activities of ALT and AST (P<0.05), to remarkably lower the liver indexes and MDA level in hepatical tissues in mice (P<0.05), and to upregulated the lower T-SOD level in liver homogenate (P<0.01). Furthermore, histologic examination showed that PYP could attenuate and the extent of necrosis, reduce the immigration of inflammatory cells. PYP plays a protective action against hepatotoxicity induced by CCl(4) in mice, and its mechanisms may be related to free radical scavenging, increasing SOD activities and anti-lipid peroxide.
为研究条斑紫菜多糖（PYP）对肝毒性小鼠的保护作用及可能机制，通过腹腔注射0.2%四氯化碳（CCl₄）成功诱导小鼠急性肝损伤。采用生化方法检测血清和肝匀浆中丙氨酸氨基转移酶（ALT）和天冬氨酸氨基转移酶（AST）水平、肝组织中丙二醛（MDA）含量、总超氧化物歧化酶（T-SOD）活性。计算肝脏指数并显微镜观察肝组织病理变化。结果发现，PYP可显著降低ALT和AST活性（P<0.05），明显降低小鼠肝组织的肝脏指数和MDA水平（P<0.05），并上调肝匀浆中降低的T-SOD水平（P<0.01）。此外，组织学检查表明，PYP可减轻坏死程度，减少炎性细胞浸润。PYP对CCl₄诱导的小鼠肝毒性具有保护作用，其机制可能与清除自由基、增加SOD活性和抗脂质过氧化有关。
