Frigero Marcelo, Dos Santos Solange Almeida, Serra Andrey Jorge, Dos Santos Monteiro Machado Caroline, Portes Leslie Andrews, Tucci Paulo José Ferreira, Silva Flavio, Leal-Junior Ernesto Cesar, de Carvalho Paulo de Tarso Camillo
Postgraduate Program in Biophotonics Applied Health Sciences, Universidade Nove de Julho (UNINOVE), Sao Paulo, SP, Brazil.
Postgraduate Program in Rehabilitation Sciences, Universidade Nove de Julho (UNINOVE), Rua Vergueiro 235, Sao Paulo, SP, Brazil.
Lasers Med Sci. 2018 Nov;33(8):1781-1790. doi: 10.1007/s10103-018-2540-7. Epub 2018 May 28.
This study aimed to determine whether photobiomodulation therapy (PBMT) in diabetic rats subjected to high-intensity exercise interferes with the expression of the oxidative stress marker in the gastrocnemius muscle. Twenty-four male Wistar rats were included in this study comprising 16 diabetic and eight control rats. The animals were allocated into three groups-control, diabetic fatigue, and diabetic PBMT fatigue groups. Diabetes was induced via the intraperitoneal administration of streptozotocin (50 mg/kg). We subsequently assessed blood lactate levels and PBMT. The animals of the diabetic fatigue group PBMT were irradiated before the beginning of the exercises, with dose of 4 J and 808 nm, were submitted to treadmill running with speed and gradual slope until exhaustion, as observed by the maximum volume of oxygen and lactate level. The animals were euthanized and muscle tissue was removed for analysis of SOD markers, including catalase (CAT), glutathione peroxidase (GPx), and 2-thiobarbituric acid (TBARS) reactive substances. CAT, SOD, and GPx activities were significantly higher in the diabetic PBMT fatigue group (p < 0.05) than in the diabetic fatigue group. Outcomes for the diabetic PBMT fatigue group were similar to those of the control group (p > 0.05), while their antioxidant enzymes were significantly higher than those of the diabetic fatigue group. PBMT mitigated the TBARS concentration (p > 0.05). PBMT may reduce oxidative stress and be an alternative method of maintaining physical fitness when subjects are unable to perform exercise. However, this finding requires further testing in clinical studies.
本研究旨在确定高强度运动的糖尿病大鼠接受光生物调节疗法（PBMT）是否会干扰腓肠肌中氧化应激标志物的表达。本研究纳入了24只雄性Wistar大鼠，其中16只为糖尿病大鼠，8只为对照大鼠。这些动物被分为三组——对照组、糖尿病疲劳组和糖尿病PBMT疲劳组。通过腹腔注射链脲佐菌素（50 mg/kg）诱导糖尿病。随后我们评估了血乳酸水平和PBMT。糖尿病疲劳组的动物在运动开始前接受PBMT照射，剂量为4 J，波长为808 nm，然后进行跑步机跑步，速度和坡度逐渐增加，直至耗尽，通过最大摄氧量和乳酸水平来观察。对动物实施安乐死后，取出肌肉组织分析超氧化物歧化酶（SOD）标志物，包括过氧化氢酶（CAT）、谷胱甘肽过氧化物酶（GPx）和2-硫代巴比妥酸（TBARS）反应性物质。糖尿病PBMT疲劳组的CAT、SOD和GPx活性显著高于糖尿病疲劳组（p < 0.05）。糖尿病PBMT疲劳组的结果与对照组相似（p > 0.05），但其抗氧化酶显著高于糖尿病疲劳组。PBMT降低了TBARS浓度（p > 0.05）。当受试者无法进行运动时，PBMT可能会减轻氧化应激，是维持身体健康的一种替代方法。然而，这一发现需要在临床研究中进一步验证。
