七鳃鳗可变淋巴细胞受体的酵母表面展示
Yeast surface display of lamprey variable lymphocyte receptors.
作者信息
Xu Gang, Tasumi Satoshi, Pancer Zeev
机构信息
Center of Marine Biotechnology, University of Maryland Biotechnology Institute, Baltimore, MD, USA.
出版信息
Methods Mol Biol. 2011;748:21-33. doi: 10.1007/978-1-61779-139-0_2.
Abstract
The variable lymphocyte receptors (VLRs) of lamprey and hagfish comprise leucine-rich repeat modules, instead of the immunoglobulin-like domain building blocks of antibodies and T-cell receptors in jawed vertebrates. Both types of vertebrate-rearranging antigen receptors are similarly diverse, with repertoires that can potentially exceed 10(14) unique receptors. In order to characterize antigen-binding properties of the VLRs, we developed a high-throughput yeast surface display platform for the isolation of monoclonal VLRs. We have isolated VLRs that specifically bind hen egg lysozyme, β-galactosidase, cholera toxin subunit B, R-phycoerythrin, and the blood group trisaccharides A and B, with binding affinities in the mid-nanomolar to mid-picomolar range. VLRs may, thus, be excellent single-chain alternatives to Ig-based antibodies for biotechnology applications.
摘要
七鳃鳗和盲鳗的可变淋巴细胞受体（VLR）由富含亮氨酸的重复模块组成，而不是有颌脊椎动物中抗体和T细胞受体的免疫球蛋白样结构域构建块。这两种脊椎动物重排抗原受体同样具有多样性，其库可能潜在地超过10（14）种独特受体。为了表征VLR的抗原结合特性，我们开发了一种用于分离单克隆VLR的高通量酵母表面展示平台。我们已经分离出了特异性结合鸡蛋清溶菌酶、β-半乳糖苷酶、霍乱毒素亚基B、R-藻红蛋白以及血型三糖A和B的VLR，其结合亲和力在中纳摩尔到中皮摩尔范围内。因此，对于生物技术应用而言，VLR可能是基于Ig的抗体的优秀单链替代品。
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本文引用的文献
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Proc Natl Acad Sci U S A. 2009 Aug 4;106(31):12891-6. doi: 10.1073/pnas.0904443106. Epub 2009 Jul 22.
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