Laboratory of Environmental Health and Food Science, Nippon Veterinary and Life Science University, Musashino, Tokyo, Japan.
Environ Toxicol. 2012 Jan;27(1):58-62. doi: 10.1002/tox.20614. Epub 2011 Jun 23.
Endocrine disruptors, especially estrogenic substances, are thought to affect the reproduction and development of animals, including humans. We therefore assessed whether bisphenol A (BPA) or estrone (E1) had any adverse effects on chick embryogenesis. Fertilized eggs of white Leghorns were obtained within 24 h after laying. Embryos were administered 0.1, 1.0, and 10.0 mM BPA, and administered 10, 100 nM, and 1 μM E1, and incubated for 48 h at 37 ± 0.5°C and >80% relative humidity with one rotation per hour. The embryos were excised, fixed in 70% ethanol and viewed under a stereomicroscope. Their morphological abnormalities and numbers of somites were recorded. There were no significant difference in the average number of somites in embryos administered BPA and controls. Abnormal embryogenesis, however, showed dose-related increases caused by BPA and E1.
内分泌干扰物，特别是雌激素物质，被认为会影响动物（包括人类）的生殖和发育。因此，我们评估了双酚 A（BPA）或雌酮（E1）是否对鸡胚发生有任何不良影响。白来航鸡的受精卵在产卵后 24 小时内获得。胚胎分别给予 0.1、1.0 和 10.0 mM BPA，以及 10、100 nM 和 1 μM E1，并在 37 ± 0.5°C 和 >80%相对湿度下孵育 48 小时，每小时旋转一次。将胚胎取出，用 70%乙醇固定，在立体显微镜下观察。记录其形态异常和体节数。给予 BPA 和对照的胚胎的平均体节数没有显著差异。然而，异常胚胎发生显示出由 BPA 和 E1 引起的剂量相关增加。
