Bak-Romaniszyn Leokadia, Szala Agnieszka, Sokolowska Anna, Mierzwa Grazyna, Czerwionka-Szaflarska Mieczyslawa, Swierzko Anna St, Zeman Krzysztof, Cedzynski Maciej
Unit of Nutrition in Digestive Tract Disease, Medical University of Lodz, Lodz, Poland.
Scand J Gastroenterol. 2011 Oct;46(10):1275-8. doi: 10.3109/00365521.2011.594087. Epub 2011 Jun 27.
The incidence of inflammatory bowel disease (IBD) in Europe has increased significantly. At least a fourth of patients are children. Mannan-binding lectin (MBL) is believed to be an important component of innate immunity, acting as an opsonin and activator of the lectin pathway (LP) of complement. The data relating any of the LP factors to IBD are sparse and contradictory and were obtained mainly from adult patients. The aim of this study was to investigate the possible role of MBL in Crohn's disease (CD) and ulcerative colitis (UC) in children.
MBL2 gene single nucleotide polymorphisms (PCR-RFLP) and MBL concentrations (ELISA) were determined.
The frequency of MBL2 gene variants responsible for MBL deficiency (LXPA/O and O/O) is significantly higher in CD patients compared with controls or children with UC. A relatively high frequency of the codon 52 mutation (D allele) was noted in these patients. Practically no difference was found between UC and control (C) groups. Similarly, the average MBL levels as well as the number of MBL-deficient (MBL concentrations < 150 ng/ml) individuals differed between CD patients and controls or children suffering from UC. Again, there was no difference between UC and C groups.
These data suggest that MBL deficiency may be associated with CD but not with UC in pediatric patients. The possible role of MBL in IBD requires confirmation in larger series and further investigation of the mechanisms involved.
欧洲炎症性肠病（IBD）的发病率显著上升。至少四分之一的患者为儿童。甘露聚糖结合凝集素（MBL）被认为是天然免疫的重要组成部分，作为补体凝集素途径（LP）的调理素和激活剂。将任何LP因子与IBD相关的数据稀少且相互矛盾，并且主要来自成年患者。本研究的目的是调查MBL在儿童克罗恩病（CD）和溃疡性结肠炎（UC）中的可能作用。
测定MBL2基因单核苷酸多态性（PCR-RFLP）和MBL浓度（ELISA）。
与对照组或UC患儿相比，CD患者中导致MBL缺乏的MBL2基因变异（LXPA/O和O/O）频率显著更高。在这些患者中观察到密码子52突变（D等位基因）的频率相对较高。UC组与对照组（C组）之间几乎没有差异。同样，CD患者与对照组或UC患儿之间的平均MBL水平以及MBL缺乏（MBL浓度<150 ng/ml）个体数量也存在差异。UC组与C组之间再次没有差异。
这些数据表明，在儿科患者中，MBL缺乏可能与CD相关，但与UC无关。MBL在IBD中的可能作用需要在更大规模的研究中得到证实，并对相关机制进行进一步研究。
