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成骨的骨形态发生蛋白2（BMP2）以及成腱的骨形态发生蛋白12（BMP12）和骨形态发生蛋白13（BMP13）的不同活性，与受体结合亲和力无关。

Divergent activities of osteogenic BMP2, and tenogenic BMP12 and BMP13 independent of receptor binding affinities.

作者信息

Berasi Stephen P, Varadarajan Usha, Archambault Joanne, Cain Michael, Souza Tatyana A, Abouzeid Abe, Li Jian, Brown Christopher T, Dorner Andrew J, Seeherman Howard J, Jelinsky Scott A

机构信息

Tissue Repair, Pfizer Research, Cambridge, MA 02140, USA.

出版信息

Growth Factors. 2011 Aug;29(4):128-39. doi: 10.3109/08977194.2011.593178. Epub 2011 Jun 27.

DOI:10.3109/08977194.2011.593178
PMID:21702718
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC3154542/
Abstract

Ectopic expression of recombinant human bone morphogenetic protein 2 (rhBMP2) induces osteogenesis, while ectopic expression of rhBMP12 and rhBMP13 induces the formation of tendon-like tissue. Despite their different in vivo activities, all three ligands bound to the type I bone morphogenic protein receptors (BMPRs), activin receptor-like kinase (ALK)-3 and ALK6, and to the type II BMPRs, activin receptor type-2A, activin receptor type-2B, and BMPR2, with similar affinities. Treatment of C3H10T1/2 cells with rhBMP2 activated SMAD signaling and induced expression of osteoblast markers including osteocalcin mRNA (Ocn). In contrast, treatment with rhBMP12 or rhBMP13 resulted in a dose-dependent induction of a tendon-specific gene (Thbs4) expression with no detectable activation of SMAD 1, 5, and 8. Differential regulation of Thbs4 and Ocn has potential utility as an in vitro biomarker for induction of tenogenic signaling. Such an assay also permits the ability to distinguish between the activities of different BMPs and may prove useful in studies on the molecular mechanisms of BMP tenogenic activity.

摘要

重组人骨形态发生蛋白2（rhBMP2）的异位表达可诱导成骨，而rhBMP12和rhBMP13的异位表达则诱导腱样组织的形成。尽管它们在体内具有不同的活性，但这三种配体与I型骨形态发生蛋白受体（BMPR）、激活素受体样激酶（ALK）-3和ALK6以及II型BMPR、激活素受体2A、激活素受体2B和BMPR2结合的亲和力相似。用rhBMP2处理C3H10T1/2细胞可激活SMAD信号通路并诱导包括骨钙素mRNA（Ocn）在内的成骨细胞标志物的表达。相比之下，用rhBMP12或rhBMP13处理会导致肌腱特异性基因（Thbs4）表达的剂量依赖性诱导，而未检测到SMAD 1、5和8的激活。Thbs4和Ocn的差异调节作为诱导成腱信号的体外生物标志物具有潜在用途。这样的检测方法还能够区分不同BMP的活性，并且可能在BMP成腱活性的分子机制研究中证明是有用的。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c70b/3154542/d36cde253f12/ggrf29-128-f6.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c70b/3154542/33c1d9d42a7b/ggrf29-128-f1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c70b/3154542/efd467580bd1/ggrf29-128-f2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c70b/3154542/ed7bd79b1062/ggrf29-128-f3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c70b/3154542/379e233664c0/ggrf29-128-f4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c70b/3154542/26840e1a29b9/ggrf29-128-f5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c70b/3154542/d36cde253f12/ggrf29-128-f6.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c70b/3154542/33c1d9d42a7b/ggrf29-128-f1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c70b/3154542/efd467580bd1/ggrf29-128-f2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c70b/3154542/ed7bd79b1062/ggrf29-128-f3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c70b/3154542/379e233664c0/ggrf29-128-f4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c70b/3154542/26840e1a29b9/ggrf29-128-f5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c70b/3154542/d36cde253f12/ggrf29-128-f6.jpg

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本文引用的文献

1
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Rheumatology (Oxford). 2010 Nov;49(11):2090-7. doi: 10.1093/rheumatology/keq072. Epub 2010 Apr 1.
2
Intricacies of BMP receptor assembly.BMP 受体组装的复杂性。
Cytokine Growth Factor Rev. 2009 Oct-Dec;20(5-6):367-77. doi: 10.1016/j.cytogfr.2009.10.022.
3
Tendon-selective genes identified from rat and human musculoskeletal tissues.
Pharmaceutics. 2022 May 8;14(5):1012. doi: 10.3390/pharmaceutics14051012.
4
The Application of Mechanical Stimulations in Tendon Tissue Engineering.机械刺激在肌腱组织工程中的应用
Stem Cells Int. 2020 Sep 24;2020:8824783. doi: 10.1155/2020/8824783. eCollection 2020.
5
In Vitro Innovation of Tendon Tissue Engineering Strategies.体外创新的肌腱组织工程策略。
Int J Mol Sci. 2020 Sep 14;21(18):6726. doi: 10.3390/ijms21186726.
6
Angelica polysaccharide promotes proliferation and osteoblast differentiation of mesenchymal stem cells by regulation of long non-coding RNA H19: An animal study.当归多糖通过调控长链非编码RNA H19促进间充质干细胞增殖和成骨细胞分化：一项动物研究
Bone Joint Res. 2019 Aug 2;8(7):323-332. doi: 10.1302/2046-3758.87.BJR-2018-0223.R2. eCollection 2019 Jul.
7
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Curr Stem Cell Res Ther. 2019;14(8):618-643. doi: 10.2174/1574888X14666190628103528.
8
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9
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10
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