酿酒酵母中硫胺素生物合成的调控
Regulation of thiamine biosynthesis in Saccharomyces cerevisiae.
作者信息
Kawasaki Y, Nosaka K, Kaneko Y, Nishimura H, Iwashima A
机构信息
Department of Biochemistry, Kyoto Prefectural University of Medicine, Japan.
出版信息
J Bacteriol. 1990 Oct;172(10):6145-7. doi: 10.1128/jb.172.10.6145-6147.1990.
Abstract
A pho6 mutant of Saccharomyces cerevisiae, lacking a regulatory gene for the synthesis of periplasmic thiamine-repressible acid phosphatase activity, was found to be auxotrophic for thiamine. The activities of four enzymes involved in the synthesis of thiamine monophosphate were hardly detectable in the crude extract from the pho6 mutant. On the other hand, the activities of these enzymes and thiamine-repressible acid phosphatase in a wild-type strain of S. cerevisiae, H42, decreased with the increase in the concentration of thiamine in yeast cells. These results suggest that thiamine synthesis in S. cerevisiae is subject to a positive regulatory gene, PHO6, whereas it is controlled negatively by the intracellular thiamine level.
摘要
酿酒酵母的一个pho6突变体，缺乏用于合成周质硫胺素可阻遏酸性磷酸酶活性的调控基因，被发现对硫胺素营养缺陷。在pho6突变体的粗提取物中，几乎检测不到参与硫胺素单磷酸合成的四种酶的活性。另一方面，酿酒酵母野生型菌株H42中这些酶的活性以及硫胺素可阻遏酸性磷酸酶的活性，随着酵母细胞中硫胺素浓度的增加而降低。这些结果表明，酿酒酵母中的硫胺素合成受一个正向调控基因PHO6的影响，而它受到细胞内硫胺素水平的负调控。
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