Pontificia Universidad Católica del Perú, Engineering Department, Lima, Peru.
Nanomedicine. 2012 Jan;8(1):37-45. doi: 10.1016/j.nano.2011.05.007. Epub 2011 Jun 2.
Synthesis of nanosized particles with antibacterial properties is of great interest in the development of new pharmaceutical products. Silver nanoparticles (Ag NPs) are known to have inhibitory and bactericidal effects. In this article we present the synthesis of Ag NPs prepared by chemical reduction from aqueous solutions of silver nitrate, containing a mixture of hydrazine hydrate and sodium citrate as reductants and sodium dodecyl sulfate as a stabilizer. The results of the characterization of the Ag NPs show agglomerates of grains with a narrow size distribution (from 40 to 60 nm), whereas the radii of the individual particles are between 10 and 20 nm. Finally, the antibacterial activity was measured by the Kirby-Bauer method. The results showed reasonable bactericidal activity against Escherichia coli, Pseudomonas aeruginosa, and Staphylococcus aureus. The standard dilution micromethod, determining the minimum inhibitory concentration leading to inhibition of bacterial growth, is still under way. Preliminary results have been obtained.
In this paper the synthesis of Ag NPs via chemical reduction from aqueous solutions is discussed. Reasonable bactericidal activity against Escherichia coli, Pseudomonas aeruginosa, and Staphylococcus aureus was demonstrated.
在开发新的医药产品中，具有抗菌性能的纳米颗粒的合成具有重要意义。已知银纳米颗粒（Ag NPs）具有抑制和杀菌作用。本文介绍了通过化学还原法从硝酸银水溶液中制备 Ag NPs 的方法，该方法使用水合肼和柠檬酸钠的混合物作为还原剂，十二烷基硫酸钠作为稳定剂。Ag NPs 的表征结果表明，颗粒的团聚体具有较窄的粒径分布（40-60nm），而单个颗粒的半径在 10-20nm 之间。最后，通过 Kirby-Bauer 法测量了抗菌活性。结果表明，对大肠杆菌、铜绿假单胞菌和金黄色葡萄球菌具有合理的杀菌活性。测定抑菌浓度以抑制细菌生长的标准稀释微量法仍在进行中。已经获得了初步结果。
本文讨论了通过化学还原法从水溶液中合成 Ag NPs。对大肠杆菌、铜绿假单胞菌和金黄色葡萄球菌表现出合理的杀菌活性。
