Department of Mechanical Engineering, Virginia Tech, Blacksburg, Virginia, USA.
Nanomedicine. 2012 Jan;8(1):93-102. doi: 10.1016/j.nano.2011.05.012. Epub 2011 Jun 23.
Alterations in the biomechanical properties and cytoskeletal organization of cancer cells in addition to genetic changes have been correlated with their aggressive phenotype. In this study, we investigated changes in the viscoelasticity of mouse ovarian surface epithelial (MOSE) cells, a mouse model for progressive ovarian cancer. We demonstrate that the elasticity of late-stage MOSE cells (0.549 ± 0.281 kPa) were significantly less than that of their early-stage counterparts (1.097 ± 0.632 kPa). Apparent cell viscosity also decreased significantly from early (144.7 ± 102.4 Pa-s) to late stage (50.74 ± 29.72 Pa-s). This indicates that ovarian cells are stiffer and more viscous when they are benign. The increase in cell deformability directly correlates with the progression of a transformed phenotype from a nontumorigenic, benign cell to a tumorigenic, malignant one. The decrease in the level of actin in the cytoskeleton and its organization is directly associated with the changes in cell biomechanical property.
The authors have investigated changes in the viscoelasticity of mouse ovarian surface epithelial (MOSE) cells and demonstrated that ovarian cells are stiffer and more viscous when they are benign.
除了遗传变化外，癌细胞的生物力学特性和细胞骨架组织的改变与它们的侵袭表型有关。在这项研究中，我们研究了小鼠卵巢表面上皮（MOSE）细胞的粘弹性变化，MOSE 细胞是进展性卵巢癌的小鼠模型。我们证明，晚期 MOSE 细胞（0.549 ± 0.281 kPa）的弹性明显小于早期 MOSE 细胞（1.097 ± 0.632 kPa）。明显的细胞粘度也从早期（144.7 ± 102.4 Pa-s）显著降低到晚期（50.74 ± 29.72 Pa-s）。这表明卵巢细胞在良性时更硬、更粘稠。细胞变形性的增加与从非致瘤性良性细胞向致瘤性恶性细胞转化的表型进展直接相关。细胞骨架中肌动蛋白水平及其组织的减少与细胞生物力学特性的变化直接相关。
作者研究了小鼠卵巢表面上皮（MOSE）细胞粘弹性的变化，证明当卵巢细胞为良性时，其更硬且更粘稠。
