Ishikawa O, LeRoy E C, Trojanowska M
Department of Medicine, Medical University of South Carolina, Charleston 29425.
J Cell Physiol. 1990 Oct;145(1):181-6. doi: 10.1002/jcp.1041450124.
Platelet-derived growth factor (PDGF) and transforming growth factor beta (TGF-beta), potent modulators of mesenchymal cell growth and differentiation, are often colocalizable in vivo. Previous in vitro studies in fibroblastic cell lines have shown variable, even antagonistic effects of TGF-beta on the mitogenic action of PDGF. This study demonstrates that in diploid human dermal fibroblasts, TGF-beta 1 is weakly mitogenic in the absence of serum or purified growth factors, and that TGF-beta 1 potentiates DNA synthesis in PDGF-stimulated fibroblasts with delayed kinetics when compared to stimulation with PDGF alone. TGF-beta 1 enhances mitogenic potency of all three PDGF isoforms and increases receptor binding of both 125I PDGF-AA and 125I PDGF-BB, consistent with the increased expression of the alpha type PDGF receptor. The induction of PDGF alpha receptor subunits by TGF-beta may play a role in enhancing the proliferative potential of human fibroblasts in certain physiologic and pathologic conditions.
血小板衍生生长因子（PDGF）和转化生长因子β（TGF-β）是间充质细胞生长和分化的强效调节剂，在体内常可共定位。先前对成纤维细胞系的体外研究表明，TGF-β对PDGF的促有丝分裂作用具有可变甚至拮抗的影响。本研究表明，在二倍体人皮肤成纤维细胞中，TGF-β1在无血清或纯化生长因子的情况下具有弱促有丝分裂作用，并且与单独用PDGF刺激相比，TGF-β1能增强PDGF刺激的成纤维细胞中的DNA合成，但其动力学延迟。TGF-β1增强了所有三种PDGF异构体的促有丝分裂效力，并增加了125I PDGF-AA和125I PDGF-BB的受体结合，这与α型PDGF受体表达增加一致。TGF-β诱导PDGFα受体亚基可能在某些生理和病理条件下增强人成纤维细胞的增殖潜能中发挥作用。
