Center for Molecular Diversity In Drug Design, Discovery, and Delivery, Department of Chemistry, University of South Florida, Tampa, FL 33620, USA.
Bioorg Med Chem Lett. 2011 Aug 1;21(15):4589-91. doi: 10.1016/j.bmcl.2011.05.104. Epub 2011 Jun 6.
We describe a method to obtain purified, polyacrylate nanoparticles in a homogeneous powdered form that can be readily reconstituted in aqueous media for in vivo applications. Polyacrylate-based nanoparticles can be easily prepared by emulsion polymerization using a 7:3 mixture of butyl acrylate and styrene in water containing sodium dodecyl sulfate as a surfactant and potassium persulfate as a water-soluble radical initiator. The resulting emulsions contain nanoparticles measuring 40-50 nm in diameter with uniform morphology, and can be purified by centrifugation and dialysis to remove larger coagulants as well as residual surfactant and monomers associated with toxicity. These purified emulsions can be lyophilized in the presence of maltose (a non-toxic cryoprotectant) to provide a homogeneous dried powder, which can be reconstituted as an emulsion by addition of an aqueous diluent. Dynamic light scattering and microbiological experiments were carried out on the reconstituted nanoparticles. This procedure allows for ready preparation of nanoparticle emulsions for drug delivery applications.
我们描述了一种方法，可以获得纯化的、以均一粉末形式存在的聚丙烯酸酯纳米粒子，这些纳米粒子可以很容易地在水介质中重新配制，用于体内应用。基于聚丙烯酸酯的纳米粒子可以通过乳液聚合很容易地制备，使用 7:3 的丁酯和苯乙烯混合物在含有十二烷基硫酸钠的水溶液中作为表面活性剂和过硫酸钾作为水溶性自由基引发剂。所得乳液含有直径为 40-50nm 的具有均匀形态的纳米粒子，并可以通过离心和透析进行纯化，以去除较大的凝聚物以及与毒性相关的残留表面活性剂和单体。这些纯化的乳液可以在麦芽糖（一种无毒的冷冻保护剂）存在下冻干，以提供均一的干粉，通过添加水性稀释剂可以重新配制为乳液。对重新配制的纳米粒子进行了动态光散射和微生物实验。该方法允许为药物输送应用方便地制备纳米粒子乳液。
