具有高超顺氧化铁包封效率、稳定性和磁性能的磁脂质体。
Magnetoliposomes with high USPIO entrapping efficiency, stability and magnetic properties.
机构信息
Laboratory of Pharmaceutical Technology, Dept. of Pharmacy, School of Health Sciences, University of Patras, Patras, Greece.
出版信息
Nanomedicine. 2011 Oct;7(5):572-9. doi: 10.1016/j.nano.2011.06.010. Epub 2011 Jun 24.
The DRV technique (followed by extrusion) was used for construction of hydrophilic-USPIO encapsulating liposomes. Magnetoliposomes (ML) were characterized for size, surface charge, entrapment, physical stability and magnetic properties (relaxivity). Results show that nanosized extruded-DRV MLs encapsulate higher amounts of USPIOs in comparison with sonicated vesicles. Fe (III) encapsulation efficiency (EE) is 12%, the highest reported to date for nanosized MLs. EE of MLs is influenced by ML membrane composition and polyethyleneglycol (PEG) coating. PEG-coating increases ML EE and stability; however, r(2)-to-r(1) ratios decrease (in comparison with non-PEGylated MLs). Most ML-types are efficient T2 contrast agents (because r(2)-to-r(1) ratios are higher than that of free USPIOs). Targeted MLs were formed by successfully immobilizing OX-26 monoclonal antibody on ML surface (biotin-streptavidin ligation), without significant loss of USPIOs. Targeted MLs retained their nanosize and integrity during storage for 1 month at 4 °C and up to 2 weeks at 37 °C.
DRV 技术（随后进行挤压）用于构建亲水性-USPIO 包封脂质体。对磁脂质体（ML）进行了大小、表面电荷、包封率、物理稳定性和磁性能（弛豫率）的表征。结果表明，与超声处理的囊泡相比，纳米挤压 DRV ML 包封了更多的 USPIO。Fe(III)包封效率（EE）为 12%，是迄今为止报道的纳米 ML 中最高的。ML 的 EE 受 ML 膜组成和聚乙二醇（PEG）涂层的影响。PEG 涂层增加了 ML 的 EE 和稳定性；然而，r(2)-to-r(1) 比值降低（与非 PEG 化 ML 相比）。大多数 ML 类型都是有效的 T2 对比剂（因为 r(2)-to-r(1) 比值高于游离 USPIO）。通过成功地将 OX-26 单克隆抗体固定在 ML 表面上（生物素-链霉亲和素连接），形成了靶向 ML，而没有明显损失 USPIO。在 4°C 下储存 1 个月和在 37°C 下储存长达 2 周期间，靶向 ML 保持其纳米尺寸和完整性。
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