Brigham & Women's Hospital, Harvard Medical School, Boston, MA, USA.
Gastrointest Endosc. 2011 Jul;74(1):189-194.e1. doi: 10.1016/j.gie.2011.03.1182.
Early recurrent hemorrhage after endoscopic intervention for acute upper GI bleeding (UGIB) can approach 20% and leads to increased morbidity and mortality. Little has changed over the past several decades regarding immediate posthemorrhage surveillance, and there has likewise been no significant improvement in outcomes.
To develop and test an endoscopically implantable wireless biosensor for real-time detection of fluorescein-labeled blood in ex vivo and in vivo porcine models of UGIB.
Animal laboratory.
Benchtop and acute animal studies.
Five pigs.
UGIB models were surgically created in living pigs. Biosensors were endoscopically deployed in the stomach using standard endoscopic clips. The ability to detect acute UGIB and estimated blood loss leading to biosensor activation were recorded. Feasibility of wireless data transmission out of the body to an external computer and cell phone was assessed.
Technical feasibility and immediate complications.
A porcine UGIB model was successfully created. Biosensors were able to detect all acute bleeding events and wirelessly transmit out of the body, and successfully sent an emergency text message to the intended cell phone in all cases. Average estimated blood loss leading to biosensor activation was 30 mL (10-75 mL).
Animal study; small numbers.
An endoscopically implantable wireless biosensor successfully detected acute hemorrhage in a porcine UGIB model and sent an emergency cell-phone alert in real time.
内镜介入治疗急性上消化道出血（UGIB）后早期再出血率接近 20%，导致发病率和死亡率增加。在出血后即时监测方面，过去几十年几乎没有任何改变，结果也没有明显改善。
开发和测试一种可内镜植入的无线生物传感器，用于实时检测荧光素标记的血液，该生物传感器在急性 UGIB 的离体和体内猪模型中进行了测试。
动物实验室。
台架和急性动物研究。
五头猪。
通过手术在活体猪中创建 UGIB 模型。使用标准内镜夹将生物传感器内镜部署在胃中。记录检测急性 UGIB 和导致生物传感器激活的估计出血量的能力。评估无线数据传输到体外的外部计算机和手机的可行性。
技术可行性和即时并发症。
成功建立了猪 UGIB 模型。生物传感器能够检测到所有急性出血事件，并能够无线传输到体外，并在所有情况下都成功地向预定的手机发送了紧急短信。导致生物传感器激活的平均估计出血量为 30 毫升（10-75 毫升）。
动物研究；数量较少。
一种可内镜植入的无线生物传感器成功地检测到了猪 UGIB 模型中的急性出血，并实时发送了紧急手机警报。
